PDF(Pubmed)
Abstract：
IgE-mediated mast cell (MC) activation is a critical component of allergic responses to oral Ags. Several T cell-derived cytokines have been shown to promote MC reactivity, and we recently demonstrated a critical role for the cytokine IL-10 in mediating MC responses during food allergy. In this study, we further validate the role of IL-10 using Ab-mediated IL-10 depletion. IL-10 neutralization significantly attenuated MC responses, leading to decreased MC accumulation and activation, as well as inhibition of MC-mediated symptoms such as allergic diarrhea. This was accompanied by decreased Th2 cytokine gene expression, attenuated systemic T cell responses, and fewer CD4 T cells, B cells, and MCs in the spleen. Our data further confirm the role of IL-10 in driving MC responses and suggest that IL-10-responsive MCs may constitute an important player in allergic responses.
摘要：
IgE介导的肥大细胞（MC）激活是对口服Ags的过敏反应的关键组成部分。一些T细胞衍生的细胞因子已被证明可以促进MC反应性，我们最近证明了细胞因子IL-10在食物过敏期间介导MC反应中的关键作用。在这项研究中,我们使用Ab介导的IL-10耗竭进一步验证了IL-10的作用.IL-10中和显著减弱MC反应，导致MC积累和激活减少，以及抑制MC介导的症状，如过敏性腹泻。这伴随着Th2细胞因子基因表达的降低，减弱的系统性T细胞反应，和更少的CD4T细胞，B细胞,和脾脏中的MC。我们的数据进一步证实了IL-10在驱动MC反应中的作用，并表明IL-10反应性MC可能在过敏反应中发挥重要作用。
