Abstract：
The majority of antibiotics are natural products, with microorganism-generated molecules and their derivatives being the most prevalent source of drugs to treat infections. Thus, identifying natural products remains the most valuable resource for novel therapeutics. Here, we report the discovery of a series of dormant bacteria in honey that have bactericidal activity toward Legionella, a bacterial pathogen that causes respiratory disease in humans. We show that, in response to bacterial products secreted by Legionella, the honey bacteria release diffusible antimicrobial molecules. Remarkably, the honey bacteria only produce these molecules in response to Legionella spp., when compared to a panel of 24 bacterial pathogens from different genera. However, the molecules induced by Legionella have broad activity against several clinically important pathogens, including many high-priority pathogens. Thus, Legionella spp. are potent drivers of antimicrobial molecule production by uncharacterized bacteria isolated from honey, providing access to new antimicrobial products and an unprecedented strategy for discovering novel antibiotics.
OBJECTIVE: Natural products generated by microorganisms remain the most viable and abundant source of new antibiotics. However, their discovery depends on the ability to isolate and culture the producing organisms and to identify conditions that promote antibiotic production. Here, we identify a series of previously undescribed bacteria isolated from raw honey and specific culture conditions that induce the production of antimicrobial molecules that are active against a wide variety of pathogenic bacteria.
OBJECTIVE: Natural products generated by microorganisms remain the most viable and abundant source of new antibiotics. However, their discovery depends on the ability to isolate and culture the producing organisms and to identify conditions that promote antibiotic production. Here, we identify a series of previously undescribed bacteria isolated from raw honey and specific culture conditions that induce the production of antimicrobial molecules that are active against a wide variety of pathogenic bacteria.
摘要：
大多数抗生素是天然产物，微生物产生的分子及其衍生物是治疗感染的最普遍的药物来源。因此，鉴定天然产物仍然是新疗法的最有价值的资源。这里,我们报道在蜂蜜中发现了一系列对军团菌具有杀菌活性的休眠细菌，引起人类呼吸道疾病的细菌病原体。我们证明,作为对军团菌分泌的细菌产物的反应，蜂蜜细菌释放可扩散的抗菌分子。值得注意的是,蜂蜜细菌仅在对军团菌的反应中产生这些分子。,当与一组来自不同属的24种细菌病原体进行比较时。然而,军团菌诱导的分子对几种临床上重要的病原体具有广泛的活性，包括许多高优先级的病原体。因此，军团菌属。是从蜂蜜中分离出的未表征细菌产生抗菌分子的有效驱动因素，提供新的抗菌产品和前所未有的战略，发现新的抗生素。
目的：由微生物产生的天然产物仍然是最可行和最丰富的新型抗生素来源。然而,它们的发现取决于分离和培养生产生物以及确定促进抗生素生产的条件的能力。这里,我们鉴定了一系列以前未描述的从生蜂蜜和特定培养条件中分离的细菌，这些细菌诱导产生抗微生物分子，这些分子对多种病原菌具有活性。
目的：由微生物产生的天然产物仍然是最可行和最丰富的新型抗生素来源。然而,它们的发现取决于分离和培养生产生物以及确定促进抗生素生产的条件的能力。这里,我们鉴定了一系列以前未描述的从生蜂蜜和特定培养条件中分离的细菌，这些细菌诱导产生抗微生物分子，这些分子对多种病原菌具有活性。
