Abstract：
BACKGROUND: The incidence of oral squamous cell carcinoma (OSCC) is increasing, and more effective treatment protocols must rapidly be developed to prevent the death of patients and ensure favorable outcomes. CircRNAs are a unique class of noncoding ribonucleic acid (RNA) molecules unaffected by RNA exonucleases. CircRNAs have more stable expression than linear RNAs and are not readily degraded; therefore, they are the newest focus of RNA research. Here, we analyze the mechanism of hsa_circ_0004771 (circ_0004771) in OSCC to provide a clinical reference.
METHODS: Circ_0004771 expression was measured in peripheral blood, cancerous tissues and adjacent tissues of OSCC patients. Patients were followed up for 3 years. The diagnostic value of circ_0004771 for OSCC occurrence, prognosis, recurrence and survival was analyzed with receiver operating characteristic (ROC) curves. OSCC cells were lentivirally transduced with a circ_0004771-silencing or an empty vector to evaluate alterations in cell growth, invasion, and apoptosis. Apoptosis-related and epithelial-mesenchymal transition (EMT)-related protein expression was quantified. BALB/c nude mice were used for tumorigenesis experiments to evaluate tumor growth in vivo after silencing circ_0004771.
RESULTS: Circ_0004771 expression was higher in peripheral blood and cancerous tissue of OSCC patients than in control peripheral blood and paracancerous tissue, respectively, exhibiting excellent predictive value for OSCC occurrence, prognosis, recurrence and survival. Silencing circ_0004771 decreased the growth, invasiveness, and EMT capacity and increased the apoptosis of OCC cells. In mice implanted with OSCC cells transduced with the circ_0004771-silencing lentiviral vector, the tumor growth capacity was obviously decreased.
CONCLUSIONS: Silencing circ_0004771 inhibits the malignant growth of OSCC.
METHODS: Circ_0004771 expression was measured in peripheral blood, cancerous tissues and adjacent tissues of OSCC patients. Patients were followed up for 3 years. The diagnostic value of circ_0004771 for OSCC occurrence, prognosis, recurrence and survival was analyzed with receiver operating characteristic (ROC) curves. OSCC cells were lentivirally transduced with a circ_0004771-silencing or an empty vector to evaluate alterations in cell growth, invasion, and apoptosis. Apoptosis-related and epithelial-mesenchymal transition (EMT)-related protein expression was quantified. BALB/c nude mice were used for tumorigenesis experiments to evaluate tumor growth in vivo after silencing circ_0004771.
RESULTS: Circ_0004771 expression was higher in peripheral blood and cancerous tissue of OSCC patients than in control peripheral blood and paracancerous tissue, respectively, exhibiting excellent predictive value for OSCC occurrence, prognosis, recurrence and survival. Silencing circ_0004771 decreased the growth, invasiveness, and EMT capacity and increased the apoptosis of OCC cells. In mice implanted with OSCC cells transduced with the circ_0004771-silencing lentiviral vector, the tumor growth capacity was obviously decreased.
CONCLUSIONS: Silencing circ_0004771 inhibits the malignant growth of OSCC.
摘要：
背景：口腔鳞状细胞癌（OSCC）的发病率正在增加，和更有效的治疗方案必须迅速发展，以防止患者死亡，并确保良好的结果。CircRNA是一类独特的非编码核糖核酸（RNA）分子，不受RNA外切核酸酶的影响。CircRNA比线性RNA具有更稳定的表达，并且不易降解；因此，它们是RNA研究的最新焦点。这里,我们分析了hsa_circ_0004771（circ_0004771）在OSCC中的作用机制，为临床提供参考。
方法：测量外周血中Circ_0004771的表达，OSCC患者的癌组织和邻近组织。患者随访3年。circ_0004771对OSCC发生的诊断价值,预后,用受试者工作特征(ROC)曲线分析复发和生存。用circ_0004771沉默或空载体慢病毒转导OSCC细胞，以评估细胞生长的改变，入侵,和凋亡。对凋亡相关和上皮间质转化(EMT)相关蛋白表达进行定量。将BALB/c裸鼠用于肿瘤发生实验以评估沉默circ_0004771后的体内肿瘤生长。
结果：Circ_0004771在OSCC患者外周血和癌组织中的表达高于对照组外周血和癌旁组织，分别,对OSCC的发生表现出极好的预测价值，预后,复发和生存。沉默circ_0004771降低了生长，侵入性,和EMT能力，并增加OCC细胞的凋亡。在植入用circ_0004771沉默慢病毒载体转导的OSCC细胞的小鼠中，肿瘤生长能力明显下降。
结论：沉默circ_0004771可抑制OSCC的恶性生长。
方法：测量外周血中Circ_0004771的表达，OSCC患者的癌组织和邻近组织。患者随访3年。circ_0004771对OSCC发生的诊断价值,预后,用受试者工作特征(ROC)曲线分析复发和生存。用circ_0004771沉默或空载体慢病毒转导OSCC细胞，以评估细胞生长的改变，入侵,和凋亡。对凋亡相关和上皮间质转化(EMT)相关蛋白表达进行定量。将BALB/c裸鼠用于肿瘤发生实验以评估沉默circ_0004771后的体内肿瘤生长。
结果：Circ_0004771在OSCC患者外周血和癌组织中的表达高于对照组外周血和癌旁组织，分别,对OSCC的发生表现出极好的预测价值，预后,复发和生存。沉默circ_0004771降低了生长，侵入性,和EMT能力，并增加OCC细胞的凋亡。在植入用circ_0004771沉默慢病毒载体转导的OSCC细胞的小鼠中，肿瘤生长能力明显下降。
结论：沉默circ_0004771可抑制OSCC的恶性生长。
