PDF(Pubmed)
Abstract：
Introduction: Facial nerve injury significantly impacts both the physical and psychological] wellbeing of patients. Despite advancements, there are still limitations associated with autografts transplantation. Consequently, there is an urgent need for effective artificial grafts to address these limitations and repair injuries. Recent years have witnessed the recognition of the beneficial effects of chitosan (CS) and graphene in the realm of nerve repair. Dental pulp stem cells (DPSCs) hold great promise due to their high proliferative and multi-directional differentiation capabilities. Methods: In this study, Graphene/CS (G/CST) composite tubes were synthesized and their physical, chemical and biological properties were evaluated, then DPSCs were employed as seed cells and G/CST as a scaffold to investigate their combined effect on promoting facial nerve injury repair. Results and Disscussion: The experimental results indicate that G/CST possesses favorable physical and chemical properties, along with good cyto-compatibility. making it suitable for repairing facial nerve transection injuries. Furthermore, the synergistic application of G/CST and DPSCs significantly enhanced the repair process for a 10 mm facial nerve defect in rabbits, highlighting the efficacy of graphene as a reinforcement material and DPSCs as a functional material in facial nerve injury repair. This approach offers an effective treatment strategy and introduces a novel concept for clinically managing facial nerve injuries.
摘要：
简介：面神经损伤显着影响患者的身体和心理]健康。尽管取得了进步，自体移植仍然存在局限性。因此,迫切需要有效的人工移植物来解决这些限制和修复损伤。近年来，人们认识到壳聚糖（CS）和石墨烯在神经修复领域的有益作用。牙髓干细胞（DPSC）由于其高增殖和多向分化能力而具有广阔的前景。方法:在本研究中,合成了石墨烯/CS(G/CST)复合管,化学和生物学特性进行了评估，然后以DPSCs为种子细胞，G/CST为支架，研究其促进面神经损伤修复的联合作用。结果与讨论：实验结果表明，G/CST具有良好的物理和化学性质，以及良好的cyto兼容性。使其适用于修复面神经横断伤。此外，G/CST和DPSCs的协同应用可显著促进兔10mm面神经缺损的修复过程,强调了石墨烯作为增强材料和DPSC作为功能材料在面神经损伤修复中的功效。这种方法提供了一种有效的治疗策略，并引入了临床治疗面神经损伤的新概念。
