PDF(Pubmed)
Abstract：
Androgenic alopecia (AGA) affects both men and women worldwide. New blood vessel formation can restore blood supply and stimulate the hair regrowth cycle. Recently, our group reported that 2-deoxy-D-ribose (2dDR) is 80%-90% as effective as VEGF in the stimulation of neovascularization in in vitro models and in a chick bioassay. In this study, we aimed to assess the effect of 2dDR on hair growth. We prepared an alginate gel containing 2dDR, polypropylene glycol, and phenoxyethanol. AGA was developed in C57BL6 mice by intraperitoneally injecting testosterone (TE). A dihydrotestosterone (DHT)-treated group was used as a negative control, a minoxidil group was used as a positive control, and we included groups treated with 2dDR gel and a combination of 2dDR and minoxidil. Each treatment was applied for 20 days. Both groups treated with 2dDR gel and minoxidil stimulated the morphogenesis of hair follicles. H&E-stained skin sections of C57BL/6 mice demonstrated an increase in length, diameter, hair follicle density, anagen/telogen ratio, diameter of hair follicles, area of the hair bulb covered in melanin, and an increase in the number of blood vessels. Masson\'s trichrome staining showed an increase in the area of the hair bulb covered in melanin. The effects of the FDA-approved drug (minoxidil) on hair growth were similar to those of 2dDR (80%-90%). No significant benefit were observed by applying a combination of minoxidil with 2dDR. We conclude that 2dDR gel has potential for the treatment of androgenic alopecia and possibly other alopecia conditions where stimulation of hair regrowth is desirable, such as after chemotherapy. The mechanism of activity of 2dDR remains to be established.
摘要：
雄激素性脱发(AGA)影响全世界的男性和女性。新血管的形成可以恢复血液供应并刺激头发再生周期。最近,我们的研究小组报告说,在体外模型和小鸡生物测定中,2-脱氧-D-核糖(2dDR)在刺激新生血管形成方面的有效率是VEGF的80%-90%.在这项研究中,我们旨在评估2dDR对头发生长的影响。我们制备了含有2dDR的藻酸盐凝胶，聚丙二醇，和苯氧乙醇.通过腹膜内注射睾酮(TE)在C57BL6小鼠中形成AGA。使用二氢睾酮（DHT）治疗组作为阴性对照，米诺地尔组用作阳性对照，我们包括使用2dDR凝胶以及2dDR和米诺地尔组合治疗的组。每个处理应用20天。用2dDR凝胶和米诺地尔处理的两组刺激毛囊的形态发生。H&E染色的C57BL/6小鼠的皮肤切片显示长度增加，直径,毛囊密度，生长期/静止期比率，毛囊的直径，被黑色素覆盖的毛球区域，血管数量的增加.Masson的三色染色显示黑色素覆盖的毛球面积增加。FDA批准的药物（米诺地尔）对头发生长的影响与2dDR相似（80％-90％）。通过应用米诺地尔与2dDR的组合没有观察到显著的益处。我们得出的结论是，2DDR凝胶具有治疗雄激素性脱发和其他可能需要刺激头发再生的脱发疾病的潜力，比如化疗后。2dDR的活性机制尚待建立。
