PDF(Pubmed)
Abstract：
Osseointegration commences with foreign body inflammation upon implant placement, where macrophages play a crucial role in the immune response. Subsequently, during the intermediate and late stages of osseointegration, mesenchymal stem cells (MSCs) migrate and initiate their osteogenic functions, while macrophages support MSCs in osteogenesis. The utilization of ferroelectric P(VDF-TrFE) covered ITO planar microelectrodes facilitated the simulation of various surface charge to investigate their effects on MSCs\' osteogenic differentiation and macrophage polarization and the results indicated a parabolic increase in the promotional effect of both with the rise in piezoelectric coefficient. Furthermore, the surface charge with a piezoelectric coefficient of -18 exhibited the strongest influence on the promotion of M1 polarization of macrophages and the promotion of MSCs\' osteogenic differentiation. The impact of macrophage polarization and MSC osteogenesis following the interaction of macrophages affected by surface charge and MSC was ultimately investigated. It was observed that macrophages affected by the surface charge of -18 piezoelectric coefficient still exerted the most profound induced osteogenic effect, validating the essential role of M1-type macrophages in the osteogenic differentiation of MSCs.
摘要：
骨整合开始于植入物放置时的异物炎症，巨噬细胞在免疫反应中起着至关重要的作用。随后，在骨整合的中期和晚期，间充质干细胞（MSCs）迁移并启动其成骨功能，而巨噬细胞支持MSCs成骨。利用铁电P（VDF-TrFE）覆盖的ITO平面微电极促进了各种表面电荷的模拟，以研究它们对MSCs成骨分化和巨噬细胞极化的影响，结果表明两者的促进作用呈抛物线增加。压电系数。此外，压电系数为-18的表面电荷对促进巨噬细胞M1极化和促进MSCs成骨分化的影响最强。最终研究了受表面电荷影响的巨噬细胞与MSC相互作用后巨噬细胞极化和MSC成骨的影响。观察到受-18压电系数的表面电荷影响的巨噬细胞仍然发挥最深刻的诱导成骨作用，验证M1型巨噬细胞在MSCs成骨分化中的重要作用。
