PDF(Pubmed)
Abstract：
Cellular movement is essential for many vital biological functions where it plays a pivotal role both at the single cell level, such as during division or differentiation, and at the macroscopic level within tissues, where coordinated migration is crucial for proper morphogenesis. It also has an impact on various pathological processes, one for all, cancer spreading. Cell migration is a complex phenomenon and diverse experimental methods have been developed aimed at dissecting and analysing its distinct facets independently. In parallel, corresponding analytical procedures and tools have been devised to gain deep insight and interpret experimental results. Here we review established experimental techniques designed to investigate specific aspects of cell migration and present a broad collection of historical as well as cutting-edge computational tools used in quantitative analysis of cell motion.
摘要：
细胞运动对于许多重要的生物学功能至关重要，在单细胞水平上都起着关键作用。例如在分裂或分化期间，在组织内的宏观层面，协调的迁移对于正确的形态发生至关重要。对各种病理过程也有影响,一个为所有人，癌症扩散。细胞迁移是一种复杂的现象，已经开发了多种实验方法，旨在独立解剖和分析其不同方面。并行,相应的分析程序和工具已经设计，以获得深刻的洞察力和解释实验结果。在这里，我们回顾了既定的实验技术，旨在研究细胞迁移的特定方面，并提出了广泛的历史以及用于细胞运动定量分析的尖端计算工具。
