PDF(Pubmed)
Abstract：
UNASSIGNED: Pseudocydonia sinensis, also known as Chinese quince, is a perennial shrub or small tree highly valued for its edibility and medicinal properties.
UNASSIGNED: This study presents the first chromosome-level genome assembly of P. sinensis, achieved using HiFi sequencing and Hi-C scaffolding technology.
UNASSIGNED: The assembly resulted in a high-quality genome of 576.39 Mb in size. The genome was anchored to 17 pseudo-chromosomes, with a contig N50 of 27.6 Mb and a scaffold N50 of 33.8 Mb. Comprehensive assessment using BUSCO, CEGMA and BWA tools indicates the high completeness and accuracy of the genome assembly. Our analysis identified 116 species-specific genes, 1196 expanded genes and 1109 contracted genes. Additionally, the distribution of 4DTv values suggests that the most recent duplication event occurred before the divergence of P. sinensis from both Chaenomeles pinnatifida and Pyrus pyrifolia.
UNASSIGNED: The assembly of this high-quality genome provides a valuable platform for the genetic breeding and cultivation of P. sinensis, as well as for the comparison of the genetic complexity of P. sinensis with other important crops in the Rosaceae family.
UNASSIGNED: This study presents the first chromosome-level genome assembly of P. sinensis, achieved using HiFi sequencing and Hi-C scaffolding technology.
UNASSIGNED: The assembly resulted in a high-quality genome of 576.39 Mb in size. The genome was anchored to 17 pseudo-chromosomes, with a contig N50 of 27.6 Mb and a scaffold N50 of 33.8 Mb. Comprehensive assessment using BUSCO, CEGMA and BWA tools indicates the high completeness and accuracy of the genome assembly. Our analysis identified 116 species-specific genes, 1196 expanded genes and 1109 contracted genes. Additionally, the distribution of 4DTv values suggests that the most recent duplication event occurred before the divergence of P. sinensis from both Chaenomeles pinnatifida and Pyrus pyrifolia.
UNASSIGNED: The assembly of this high-quality genome provides a valuable platform for the genetic breeding and cultivation of P. sinensis, as well as for the comparison of the genetic complexity of P. sinensis with other important crops in the Rosaceae family.
摘要：
■中华假单胞菌，也被称为中国木瓜，是多年生灌木或小树，因其可食性和药用特性而备受重视。
■本研究首次提出了中华黄牛的染色体级基因组组装，使用HiFi测序和Hi-C支架技术实现。
■组装产生了大小为576.39Mb的高质量基因组。基因组锚定在17个假染色体上，重叠群N50为27.6Mb，支架N50为33.8Mb。使用BUSCO进行综合评估，CEGMA和BWA工具表明基因组组装的高度完整性和准确性。我们的分析确定了116种特定基因，1196个扩展基因和1109个收缩基因。此外,4DTv值的分布表明，最近的重复事件发生在中华毛节虫和Pyruspyrifolia的分化之前。
■这种高质量基因组的组装为中华黄连的遗传育种和栽培提供了宝贵的平台，以及与玫瑰科其他重要作物的遗传复杂性的比较。
■本研究首次提出了中华黄牛的染色体级基因组组装，使用HiFi测序和Hi-C支架技术实现。
■组装产生了大小为576.39Mb的高质量基因组。基因组锚定在17个假染色体上，重叠群N50为27.6Mb，支架N50为33.8Mb。使用BUSCO进行综合评估，CEGMA和BWA工具表明基因组组装的高度完整性和准确性。我们的分析确定了116种特定基因，1196个扩展基因和1109个收缩基因。此外,4DTv值的分布表明，最近的重复事件发生在中华毛节虫和Pyruspyrifolia的分化之前。
■这种高质量基因组的组装为中华黄连的遗传育种和栽培提供了宝贵的平台，以及与玫瑰科其他重要作物的遗传复杂性的比较。
