UNASSIGNED: HaCaT cells were cultured to verify the effect of early short-term hypoxia on migration through cell scratch assays. Lentiviruses with silenced or overexpressed CCL2 were used to explore the relationship between CCL2 and migration under short-term hypoxia. An acute full-thickness cutaneous wound rat model was established with the application of an ERK inhibitor to reveal the hidden role of the ERK1/2 pathway in the early stage of wound healing. The EMT process was verified in all the above experiments through western blotting.
UNASSIGNED: In our study, we found that short-term hypoxia promoted cell migration. Mechanistically, hypoxia promoted cell migration through mediating CCL2. Overexpression of CCL2 via lentivirus promoted cell migration, while silencing CCL2 via lentivirus inhibited cell migration and the production of related downstream proteins. In addition, we found that CCL2 was enriched in the ERK1/2 pathway, and the application of an ERK inhibitor in vivo and in vitro verified the upstream and downstream relationships between the CCL2 pathway and ERK1/2. Western blot results both in vivo and in vitro demonstrated that early short-term hypoxia promotes epidermal cell migration by activating the CCL2-ERK1/2 pathway and EMT during wound healing.
UNASSIGNED: Our work demonstrated that hypoxia in the early stage serves as a stimulus for triggering wound healing through activating the CCL2-ERK1/2 pathway and EMT, which promote epidermal cell migration and accelerate wound closure. These findings provide additional detailed insights into the mechanism of wound healing and new targets for clinical treatment.
■培养HaCaT细胞,以通过细胞划痕测定法验证早期短期缺氧对迁移的影响。使用沉默或过表达CCL2的慢病毒来探索CCL2与短期缺氧下迁移之间的关系。应用ERK抑制剂建立急性全层皮肤创面大鼠模型,揭示ERK1/2通路在创面愈合早期的隐藏作用。在所有上述实验中通过蛋白质印迹验证了EMT过程。
■在我们的研究中,我们发现短期缺氧促进细胞迁移。机械上,缺氧通过介导CCL2促进细胞迁移。通过慢病毒过表达CCL2促进细胞迁移,而通过慢病毒沉默CCL2抑制细胞迁移和相关下游蛋白的产生。此外,我们发现CCL2在ERK1/2通路中富集,和ERK抑制剂在体内和体外的应用验证了CCL2途径与ERK1/2之间的上游和下游关系。体内和体外的Western印迹结果表明,早期短期缺氧通过激活伤口愈合期间的CCL2-ERK1/2途径和EMT来促进表皮细胞迁移。
■我们的工作表明,早期缺氧通过激活CCL2-ERK1/2途径和EMT作为触发伤口愈合的刺激,促进表皮细胞迁移并加速伤口闭合。这些发现为伤口愈合的机制和临床治疗的新目标提供了更详细的见解。