PDF(Pubmed)
Abstract：
OBJECTIVE: To compare the staining quality between rapid hematoxylin and eosin (H&E) staining and routine H&E staining of frozen breast tissue sections.
METHODS: In this cross-sectional observational study, 120 frozen breast tissue sections were randomly assigned to rapid or routine H&E staining (n = 60 per group). Rapid H&E staining used a 7:1 mixture of modified Gill\'s hematoxylin and alcohol-soluble 1% eosin Y. The staining quality of each section was evaluated and scored. A score of >7 was considered excellent, a score of 6 to 7 good, and a score of ≤5 poor.
RESULTS: The staining time for rapid staining was approximately 3 minutes, whereas that of routine staining was approximately 12 minutes. There were no significant differences in the staining quality scores or proportions of sections in each grade between the two staining methods. The proportions of sections that were classified as excellent or good were 96.7% and 98.3% for rapid and routine staining, respectively.
CONCLUSIONS: In frozen breast tissue sections, rapid H&E staining may provide staining quality that is comparable to that of routine staining, while markedly reducing the staining time.
METHODS: In this cross-sectional observational study, 120 frozen breast tissue sections were randomly assigned to rapid or routine H&E staining (n = 60 per group). Rapid H&E staining used a 7:1 mixture of modified Gill\'s hematoxylin and alcohol-soluble 1% eosin Y. The staining quality of each section was evaluated and scored. A score of >7 was considered excellent, a score of 6 to 7 good, and a score of ≤5 poor.
RESULTS: The staining time for rapid staining was approximately 3 minutes, whereas that of routine staining was approximately 12 minutes. There were no significant differences in the staining quality scores or proportions of sections in each grade between the two staining methods. The proportions of sections that were classified as excellent or good were 96.7% and 98.3% for rapid and routine staining, respectively.
CONCLUSIONS: In frozen breast tissue sections, rapid H&E staining may provide staining quality that is comparable to that of routine staining, while markedly reducing the staining time.
摘要：
目的：比较快速苏木精-伊红(H&E)染色与常规H&E染色对冷冻乳腺组织切片的染色质量。
方法：在这项横断面观察研究中，将120个冷冻乳腺组织切片随机分配到快速或常规H&E染色(每组n=60)。快速H&E染色使用改良Gill's苏木精和醇溶性1%曙红Y的7:1混合物。对每个切片的染色质量进行评估和评分。分数>7被认为是优秀的，6到7分不错，和≤5分较差。
结果：快速染色的染色时间约为3分钟，而常规染色约为12分钟。两种染色方法之间的染色质量评分或切片比例在每个等级中没有显着差异。快速和常规染色被分类为优良或优良的切片比例分别为96.7%和98.3%，分别。
结论：在冷冻乳腺组织切片中，快速H&E染色可以提供与常规染色相当的染色质量，同时显著减少染色时间。
方法：在这项横断面观察研究中，将120个冷冻乳腺组织切片随机分配到快速或常规H&E染色(每组n=60)。快速H&E染色使用改良Gill's苏木精和醇溶性1%曙红Y的7:1混合物。对每个切片的染色质量进行评估和评分。分数>7被认为是优秀的，6到7分不错，和≤5分较差。
结果：快速染色的染色时间约为3分钟，而常规染色约为12分钟。两种染色方法之间的染色质量评分或切片比例在每个等级中没有显着差异。快速和常规染色被分类为优良或优良的切片比例分别为96.7%和98.3%，分别。
结论：在冷冻乳腺组织切片中，快速H&E染色可以提供与常规染色相当的染色质量，同时显著减少染色时间。
