PDF(Pubmed)
Abstract：
BACKGROUND: Acer truncatum Bunge is an economic, ecological, oil, and medicinal tree, and its kernel oil is rich in nervonic acid. It is crucial to explore the transcriptional expression patterns of genes affecting fatty acid synthesis to improve the quality of Acer truncatum oil.
RESULTS: This study used the seeds from high fatty acid strain YQC and those from low fatty acid strain Y38 as the test materials. Specifically, we performed a comparative transcriptome analysis of Y38 seeds and YQC to identify differentially expressed genes (DEGs) at two time points (seeds 30 days after the blooming period and 90 days after the blooming period). Compared with YQC_1 (YQC seeds at 30 days after the blooming period), a total of 3,618 DEGs were identified, including 2,333 up-regulated and 1,285 downregulated DEGs in Y38_1 (Y38 seeds at 30 days after blooming period). In the Y38_2 (Y38 seeds at 90 days after the blooming period) versus YQC_2 (YQC seeds at 90 days after the blooming period) comparison group, 9,340 genes were differentially expressed, including 5,422 up-regulated and 3,918 down-regulated genes. The number of DEGs in Y38 compared to YQC was significantly higher in the late stages of seed development. Gene functional enrichment analyses showed that the DEGs were mainly involved in the fatty acid biosynthesis pathway. And two fatty acid synthesis-related genes and seven nervonic acid synthesis-related genes were validated by qRT-PCR.
CONCLUSIONS: This study provides a basis for further research on biosynthesizing fatty acids and nervonic acidnervonic acids in A. truncatum seeds.
RESULTS: This study used the seeds from high fatty acid strain YQC and those from low fatty acid strain Y38 as the test materials. Specifically, we performed a comparative transcriptome analysis of Y38 seeds and YQC to identify differentially expressed genes (DEGs) at two time points (seeds 30 days after the blooming period and 90 days after the blooming period). Compared with YQC_1 (YQC seeds at 30 days after the blooming period), a total of 3,618 DEGs were identified, including 2,333 up-regulated and 1,285 downregulated DEGs in Y38_1 (Y38 seeds at 30 days after blooming period). In the Y38_2 (Y38 seeds at 90 days after the blooming period) versus YQC_2 (YQC seeds at 90 days after the blooming period) comparison group, 9,340 genes were differentially expressed, including 5,422 up-regulated and 3,918 down-regulated genes. The number of DEGs in Y38 compared to YQC was significantly higher in the late stages of seed development. Gene functional enrichment analyses showed that the DEGs were mainly involved in the fatty acid biosynthesis pathway. And two fatty acid synthesis-related genes and seven nervonic acid synthesis-related genes were validated by qRT-PCR.
CONCLUSIONS: This study provides a basis for further research on biosynthesizing fatty acids and nervonic acidnervonic acids in A. truncatum seeds.
摘要：
背景：元宝宏碁是一种经济，生态,油,和药用树，它的果仁油富含神经酸。探索影响脂肪酸合成的基因转录表达模式对提高元宝枫油品质至关重要。
结果:本研究使用来自高脂肪酸菌株YQC的种子和来自低脂肪酸菌株Y38的种子作为测试材料。具体来说,我们对Y38种子和YQC进行了比较转录组分析，以鉴定两个时间点（开花期后30天和开花期后90天的种子）的差异表达基因（DEGs）。与YQC_1（开花期后30天的YQC种子）相比，总共确定了3618个DEG，包括Y38_1中的2,333个上调和1,285个下调的DEG（开花期后30天的Y38种子）。在Y38_2(开花期后90天的Y38种子)与YQC_2(开花期后90天的YQC种子)对比组中，9340个基因差异表达,包括5,422个上调基因和3,918个下调基因。与YQC相比，Y38中的DEGs数量在种子发育的后期明显更高。基因功能富集分析表明，DEGs主要参与脂肪酸生物合成途径。并通过qRT-PCR验证了两个脂肪酸合成相关基因和七个神经酸合成相关基因。
结论：本研究为进一步研究元宝种子中脂肪酸和神经酸的生物合成提供了基础。
结果:本研究使用来自高脂肪酸菌株YQC的种子和来自低脂肪酸菌株Y38的种子作为测试材料。具体来说,我们对Y38种子和YQC进行了比较转录组分析，以鉴定两个时间点（开花期后30天和开花期后90天的种子）的差异表达基因（DEGs）。与YQC_1（开花期后30天的YQC种子）相比，总共确定了3618个DEG，包括Y38_1中的2,333个上调和1,285个下调的DEG（开花期后30天的Y38种子）。在Y38_2(开花期后90天的Y38种子)与YQC_2(开花期后90天的YQC种子)对比组中，9340个基因差异表达,包括5,422个上调基因和3,918个下调基因。与YQC相比，Y38中的DEGs数量在种子发育的后期明显更高。基因功能富集分析表明，DEGs主要参与脂肪酸生物合成途径。并通过qRT-PCR验证了两个脂肪酸合成相关基因和七个神经酸合成相关基因。
结论：本研究为进一步研究元宝种子中脂肪酸和神经酸的生物合成提供了基础。
