PDF(Pubmed)
Abstract：
Non-photochemical quenching (NPQ) is a protective mechanism for dissipating excess energy generated during photosynthesis in the form of heat. The accelerated relaxation of the NPQ in fluctuating light can lead to an increase in the yield and dry matter productivity of crops. Since the measurement of NPQ is time-consuming and requires specific light conditions, theoretical NPQ (NPQ(T)) was introduced for rapid estimation, which could be suitable for High-throughput Phenotyping. We investigated the potential of NPQ(T) to be used for testing plant genetic resources of chickpea under drought stress with non-invasive High-throughput Phenotyping complemented with yield traits. Besides a high correlation between the hundred-seed-weight and the Estimated Biovolume, significant differences were observed between the two types of chickpea desi and kabuli for Estimated Biovolume and NPQ(T). Desi was able to maintain the Estimated Biovolume significantly better under drought stress. One reason could be the effective dissipation of excess excitation energy in photosystem II, which can be efficiently measured as NPQ(T). Screening of plant genetic resources for photosynthetic performance could take pre-breeding to a higher level and can be implemented in a variety of studies, such as here with drought stress or under fluctuating light in a High-throughput Phenotyping manner using NPQ(T).
摘要：
非光化学猝灭（NPQ）是一种保护机制，用于以热的形式耗散光合作用过程中产生的多余能量。NPQ在波动光下的加速松弛可导致作物的产量和干物质生产率的增加。由于NPQ的测量耗时且需要特定的光照条件，引入理论NPQ(NPQ(T))进行快速估算，这可能适用于高通量表型。我们研究了NPQ（T）用于测试干旱胁迫下鹰嘴豆植物遗传资源的潜力，该方法具有非侵入性高通量表型，并补充了产量性状。除了百粒种子重量和估计的生物体积之间的高度相关性，两种鹰嘴豆和kabuli的估计生物体积和NPQ（T）之间存在显着差异。在干旱胁迫下，Desi能够更好地维持估计的生物体积。一个原因可能是光系统II中过量激发能量的有效耗散，可以有效地测量为NPQ(T)。植物光合性能遗传资源的筛选可以将预育种提高到更高的水平，并可以在各种研究中实施，例如在干旱胁迫下或在波动光照下以使用NPQ(T)的高通量表型方式。
