关键词: Biofabrication Bioink Bioprinting Extrusion Modeling Rheology

Mesh : Bioprinting / methods Tissue Engineering / methods Hydrogels / chemistry Printing, Three-Dimensional Rheology Humans Tissue Scaffolds / chemistry Viscosity

来  源:   DOI:10.1038/s41598-024-64039-y   PDF(Pubmed)

Abstract:
In the context of tissue engineering, biofabrication techniques are employed to process cells in hydrogel-based matrices, known as bioinks, into complex 3D structures. The aim is the production of functional tissue models or even entire organs. The regenerative production of biological tissues adheres to a multitude of criteria that ultimately determine the maturation of a functional tissue. These criteria are of biological nature, such as the biomimetic spatial positioning of different cell types within a physiologically and mechanically suitable matrix, which enables tissue maturation. Furthermore, the processing, a combination of technical procedures and biological materials, has proven highly challenging since cells are sensitive to stress, for example from shear and tensile forces, which may affect their vitality. On the other hand, high resolutions are pursued to create optimal conditions for subsequent tissue maturation. From an analytical perspective, it is prudent to first investigate the printing behavior of bioinks before undertaking complex biological tests. According to our findings, conventional shear rheological tests are insufficient to fully characterize the printing behavior of a bioink. For this reason, we have developed optical methods that, complementarily to the already developed tests, allow for quantification of printing quality and further viscoelastic modeling of bioinks.
摘要:
在组织工程的背景下,生物制造技术用于处理基于水凝胶的基质中的细胞,被称为生物墨水,变成复杂的3D结构。目的是产生功能性组织模型或甚至整个器官。生物组织的再生生产遵循最终决定功能组织成熟的众多标准。这些标准是生物学性质的,例如不同细胞类型在生理和机械上合适的基质中的仿生空间定位,使组织成熟。此外,处理,技术程序和生物材料的结合,由于细胞对压力敏感,因此具有很大的挑战性,例如来自剪切力和拉力,这可能会影响他们的活力。另一方面,追求高分辨率,为随后的组织成熟创造最佳条件。从分析的角度来看,在进行复杂的生物测试之前,首先调查生物墨水的打印行为是谨慎的。根据我们的发现,传统的剪切流变测试不足以完全表征生物墨水的印刷行为。出于这个原因,我们开发了光学方法,与已经开发的测试互补,允许对打印质量进行量化,并进一步对生物墨水进行粘弹性建模。
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