Abstract：
The Orthoflavivirus ilheusense (ILHV) is an arbovirus that was first isolated in Brazil in 1944 during an epidemiologic investigation of yellow fever. Is a member of the Flaviviridae family and it belongs to the antigenic complex of the Ntaya virus group. Psorophora ferox is the primary vector of ILHV and this study presents the isolation and phylogenetic analysis of ILHV in a pool of Ps. ferox collected in the state of Goiás in 2021. Viral isolation tests were performed on Vero cells and C6/36 clones. The indirect immunofluorescence test (IFI) was used to confirm the positivity of the sample. The positive sample underwent RT-qPCR, sequencing, and phylogenetic analysis. This is the first report of ILHV circulation in this municipality and presented close relationship between this isolate and another ILHV isolate collected in the city of Belém (PA).
摘要：
正黄病毒（ILHV）是一种虫媒病毒，于1944年在黄热病的流行病学调查中首次在巴西分离。是黄病毒科的成员，属于Ntaya病毒组的抗原复合物。Psorophora是ILHV的主要载体，本研究提供了Ps库中ILHV的分离和系统发育分析。ferox于2021年在Goiás州收集。对Vero细胞和C6/36克隆进行病毒分离试验。使用间接免疫荧光测试(FI)来确认样品的阳性。阳性样本接受RT-qPCR,测序,测序和系统发育分析。这是该市ILHV循环的第一份报告，并介绍了该隔离物与贝伦市（PA）收集的另一种ILHV隔离物之间的密切关系。
