Abstract：
Recent studies have shown that carbon nanotubes display good potential in tumor photothermal therapy. In this study, we aimed to investigate the therapeutic potential of nano-titanium oxide-coated multi-walled carbon nanotubes (MCNTs) against colorectal cancer (CRC). Firstly, we modified TiO2 nanosheets on the surface of MCNTs to obtain nano-TiO2-coated MCNTs. Next, we conducted cell compatibility validation on nano-TiO2-coated MCNTs, and found that nano-TiO2-coated MCNTs were safe within a certain concentration range (0∼200 μg/ml). Interestingly, nano-TiO2-coated MCNTs displayed a good killing effect in CRC cells under NIR laser irradiation. Subsequently, nano-TiO2-coated MCNTs markedly promoted the proapoptotic effects of NIR laser irradiation, and significantly inhibited the expression of cell cycle proteins CCNA1 and CCND1 in CRC cells under NIR laser irradiation, which indicated that nano-TiO2-coated MCNTs exerted anti-CRC effects under NIR laser irradiation by regulating cell apoptosis and cell cycle. Furthermore, nano-TiO2-coated MCNTs accelerated inhibitory effects on the AKT signaling pathway under NIR laser irradiation. Finally, a cell line-derived xenograft model was established, and the results showed that nano-TiO2-coated MCNTs significantly exhibited superior tumor-killing ability under NIR laser irradiation in vivo. Collectively, our results demonstrate that nano-TiO2-coated MCNTs with NIR laser irradiation may serve as an effective strategy for the treatment of CRC. This article is protected by copyright. All rights reserved.
摘要：
最近的研究表明，碳纳米管在肿瘤光热治疗中显示出良好的潜力。在这项研究中,我们旨在研究纳米氧化钛涂层多壁碳纳米管(MCNTs)对结直肠癌(CRC)的治疗潜力.首先,我们在MCNTs的表面上修饰TiO2纳米片，以获得纳米TiO2包覆的MCNTs。接下来,我们对纳米TiO2包覆的MCNTs进行了细胞相容性验证，发现纳米TiO2包覆的碳纳米管在一定浓度范围(0~200μg/ml)内是安全的。有趣的是,纳米TiO2包覆的MCNTs在近红外激光照射下对CRC细胞具有良好的杀伤作用。随后，纳米TiO2涂层MCNTs显著促进近红外激光照射的促凋亡作用,并显著抑制NIR激光照射下CRC细胞中细胞周期蛋白CCNA1和CCND1的表达,这表明纳米TiO2包覆的MCNTs在近红外激光照射下通过调节细胞凋亡和细胞周期发挥抗CRC作用。此外，纳米TiO2包覆的MCNTs在近红外激光照射下加速对AKT信号通路的抑制作用。最后,建立了细胞系来源的异种移植模型，结果表明，纳米TiO2涂层的MCNTs在近红外激光照射下在体内表现出优异的肿瘤杀伤能力。总的来说，我们的结果表明，NIR激光照射纳米TiO2包覆的MCNTs可以作为治疗CRC的有效策略。本文受版权保护。保留所有权利。
