Abstract：
As a key component of cell-cultured fish, fish skin gelatin (FSG)-based cell scaffold provides support structures for cell growth, proliferation, and differentiation. However, there are potential allergenicity risks contained in FSG-based scaffolds. In this study, 3D edible scaffolds were prepared by phase separation method and showed a contact angle of less than 90°, which indicated that the scaffolds were favorable for cell adhesion. Besides, the swelling ratio was greater than 200%, implying a great potential to support cell growth. The sequence homology analysis indicated that FSG was prone to cross-reaction with collagen analogues. Additionally, a food allergic model was constructed and represented that mice gavaged with cod FSG exhibited higher levels of specific antibodies, mast cell degranulation, vascular permeability, and intestinal barrier impairment than those gavaged with pangasius and tilapias FSG. Its higher allergenicity might be attributed to a higher number of digestion-resistant linear epitopes. Moreover, the higher hydrolysis degree linked to the exposure of linear epitopes to promote the combination with IgE, which was also responsible for maintaining the higher allergenicity of cod FSG. This study clarifies allergenic risks in cell-cultured fish and further study will focus on the allergenicity reduction of FSG-based cell scaffolds.
摘要：
作为细胞培养鱼的关键成分，基于鱼皮明胶（FSG）的细胞支架为细胞生长提供支撑结构，扩散,和差异化。然而,基于FSG的支架存在潜在的致敏性风险。在这项研究中,通过相分离法制备3D可食用支架，其接触角小于90°，这表明支架有利于细胞的粘附。此外,溶胀率大于200%,暗示着支持细胞生长的巨大潜力。序列同源性分析表明FSG易于与胶原类似物发生交叉反应。此外,建立了食物过敏模型，表明用鳕鱼FSG灌胃的小鼠表现出更高水平的特异性抗体，肥大细胞脱颗粒,血管通透性,和肠屏障损伤比那些用pangasius和tilapiasFSG灌胃的肠屏障损伤。其较高的变应原性可能归因于较高数量的耐消化线性表位。此外，较高的水解程度与线性表位的暴露有关，以促进与IgE的结合，这也是维持鳕鱼FSG较高的致敏性的原因。这项研究阐明了细胞培养鱼的致敏性风险，进一步的研究将集中在基于FSG的细胞支架的致敏性降低上。
