Abstract：
Puccinia striiformis f. sp. tritici (Pst) is a destructive pathogen that causes wheat stripe rust worldwide. Understanding the population structure and dynamic of pathogen spread is critical to fight against this disease. Limited information is available for the population genetic structure of Pst in Uzbekistan, Central Asia. In this study, we carried out surveillance from 9 different regions (Andijan, Fergana, Jizzakh, Kashkadarya, Namangan, Samarkand, Sirdaryo, Surkhandarya and Tashkent) of Uzbekistan to fill this gap. A total of 255 isolates were collected, which were genotyped using 17 polymorphic simple sequence repeats (SSR) markers. The DAPC analysis results showed no population subdivision in these sample-collected regions except Surkhandarya. Multilocus genotype (MLG) analysis, FST, and Nei\'s genetic distance results indicated a clonal population (rBarD ≤ 0.12) and merely three MLGs accounting for 70% of the overall population. MLG-34 was predominant in all Uzbekistan regions, followed by MLG-36 and MLG-42. Low genotypic diversity was observed in Andijan, Fergana, Jizzakh, Kashkadarya, Namangan, Sirdaryo, and Tashkent (0.56 to 0.76), compared with Samarkand (0.82) and Surkhandarya (0.97). No virulence against Yr5, Yr15, YrSp, and Yr26 was found, while resistant was overcome against Yr1, Yr2, Yr6, Yr9, Yr17, and Yr44 genes (Virulence frequency =≥75%). Comparative study results of Uzbekistan with previous Himalayan population were showed divergence from China and Pakistan populations. Further studies need to be conducted in a worldwide context to understand migration patterns; for that purpose, collaborative work is essential due to the Pst long-distance migration capability.
摘要：
条锈病f.sp.小麦（Pst）是一种破坏性病原体，在世界范围内引起小麦条锈病。了解病原体传播的种群结构和动态对于抗击这种疾病至关重要。关于乌兹别克斯坦Pst种群遗传结构的信息有限，中亚。在这项研究中,我们从9个不同地区进行了监视(安集延，Fergana,Jizzakh,Kashkadarya,Namangan,撒马尔罕,Sirdaryo,乌兹别克斯坦的Surkhandarya和Tashkent）填补了这一空白。总共收集了255个分离株，使用17个多态性简单序列重复（SSR）标记进行基因分型。DAPC分析结果显示，除Surkhandarya外，这些样本收集区域中没有人口细分。多位点基因型（MLG）分析，FST,Nei\的遗传距离结果表明有一个克隆种群（rBarD≤0.12），只有三个MLG占总人口的70％。MLG-34在乌兹别克斯坦所有地区都占主导地位，其次是MLG-36和MLG-42。在安集延观察到低基因型多样性，Fergana,Jizzakh,Kashkadarya,Namangan,Sirdaryo,和塔什干（0.56至0.76），与撒马尔罕（0.82）和Surkhandarya（0.97）相比。对Yr5，Yr15，YrSp没有毒力，YR26被发现了，而对Yr1，Yr2，Yr6，Yr9，Yr17和Yr44基因的抗性被克服（毒力频率≥75％）。乌兹别克斯坦与以前的喜马拉雅人口的比较研究结果表明，与中国和巴基斯坦人口存在差异。需要在世界范围内进行进一步的研究，以了解移徙模式；为此，由于Pst的长距离迁移能力，协同工作至关重要。
