Abstract：
Massive sequencing of a fragment of 16S rRNA gene allows the characterization of bacterial communities in different body sites: the microbiota. Nasal microbiota can be analyzed by DNA extraction from nasal swabs, amplification of the specific fragment of interest, and posterior sequencing. The raw sequences obtained need to go through a computational process to check their quality and then assign the taxonomy. Here, we will describe the complete process from sampling to get the microbial diversity of nasal microbiota in health and disease.
摘要：
16SrRNA基因片段的大规模测序可以表征不同身体部位的细菌群落：微生物群。可以通过从鼻拭子中提取DNA来分析鼻腔微生物群，感兴趣的特定片段的扩增，和后验测序。获得的原始序列需要经过计算过程来检查它们的质量，然后分配分类法。这里,我们将描述从采样到获得健康和疾病中鼻腔微生物群的微生物多样性的完整过程。
