Abstract：
Fatty acid-binding protein 4 (FABP4), a fatty acid transporter that coordinates lipid metabolism, is reported to exert a tumorigenic role in certain cancers. We investigated the effects of FABP4 in the carcinogenesis of thyroid cancer. Bioinformatics data about FABP4 in thyroid cancer were collected from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA). Sixteen paired papillary thyroid cancer (PTC) tissues from Taipei Medical University (TMU) were gathered, and commercial thyroid cancer complementary (c)DNA and tissue arrays were purchased to measure FABP4 messenger (m)RNA and protein levels. By analyzing data from the GEO and TCGA, we showed that FABP4 mRNA was reduced in PTC and follicular thyroid carcinoma (FTC). In addition, a lower FABP4 mRNA level in PTC was associated with poor clinical parameters and outcomes in the TCGA database. Moreover, FABP4 transcripts and proteins were downregulated in PTC and FTC, and its mRNA expression was associated with PTC staging in clinical specimens. In the TCGA database and TMU cohort, FABP4 mRNA levels were associated with thyroglobulin (r = 0.511 and r = 0.656, respectively), thyroid peroxidase (r = 0.612 and r = 0.909, respectively), and sodium iodide symporter (r = 0.485 and r = 0.637, respectively) transcripts. In conclusion, FABP4 mRNA and protein levels were reduced in PTC and FTC, and may be used as a potential indicator for thyroid cancer evolution in clinical settings. Further, well-designed research to dissect the molecular mechanism of FABP4 in modulating thyroid carcinogenesis is needed.
摘要：
脂肪酸结合蛋白4(FABP4),一种协调脂质代谢的脂肪酸转运蛋白，据报道在某些癌症中发挥致瘤作用。我们研究了FABP4在甲状腺癌癌变中的作用。从基因表达综合(GEO)和癌症基因组图谱(TCGA)收集甲状腺癌中关于FABP4的生物信息学数据。收集了来自台北医科大学（TMU）的16对乳头状甲状腺癌（PTC）组织，购买商业甲状腺癌互补(c)DNA和组织阵列以测量FABP4信使(m)RNA和蛋白质水平。通过分析GEO和TCGA的数据，我们显示PTC和滤泡性甲状腺癌（FTC）中FABP4mRNA降低。此外,PTC中较低的FABP4mRNA水平与TCGA数据库中较差的临床参数和结局相关.此外，FABP4转录本和蛋白质在PTC和FTC中下调，其mRNA表达与临床标本PTC分期有关。在TCGA数据库和TMU队列中，FABP4mRNA水平与甲状腺球蛋白相关(分别为r=0.511和r=0.656),甲状腺过氧化物酶（分别为r=0.612和r=0.909），和碘化钠转运体（分别为r=0.485和r=0.637）转录本。总之,PTC和FTC中FABP4mRNA和蛋白水平降低，并可用作临床甲状腺癌演变的潜在指标。Further,需要精心设计的研究来剖析FABP4调节甲状腺癌发生的分子机制。
