Abstract：
Ulcerative colitis (UC) is a chronic immune-mediated disease that affects the entire colon and rectum with a relapsing and remitting course, causing lifelong morbidity. When medical treatment is ineffective, especially in cases of massive gastrointestinal bleeding, perforation, toxic megacolon, or carcinogenesis, surgery becomes the last line of defense to cure UC. Total colorectal resection and ileal pouch-anal anastomosis (IPAA) offer the best chance for long-term treatment. Pouchitis is the most common and troublesome postoperative complication. In this investigation, microsurgery is employed to create an ileal pouch model in experimental rats via IPAA surgery. Subsequently, a sustained rat model of pouchitis is established by inducing inflammation of the ileal pouch with dextran sulfate sodium (DSS). The successful establishment of rat pouchitis is validated through analysis of postoperative general status, weight, food and water intake, fecal data, as well as pouch tissue pathology, immunohistochemistry, and inflammatory factor analysis. This experimental animal model of pouchitis provides a foundation for studying the pathogenesis and treatment of the condition.
摘要：
溃疡性结肠炎（UC）是一种慢性免疫介导的疾病，以复发和缓解的过程影响整个结肠和直肠，导致终身发病。当医疗无效时，尤其是在大量胃肠道出血的情况下，穿孔,有毒的巨结肠，或致癌作用，手术成为治愈UC的最后一道防线。全结直肠切除术和回肠袋肛门吻合术（IPAA）为长期治疗提供了最佳机会。囊炎是最常见和最麻烦的术后并发症。在这次调查中,显微手术用于通过IPAA手术在实验大鼠中创建回肠袋模型。随后，通过用葡聚糖硫酸钠（DSS）诱导回肠囊的炎症，建立了持续的大鼠囊炎模型。通过对术后一般情况的分析，验证了大鼠囊炎的成功建立，体重,食物和水的摄入，粪便数据,以及囊袋组织病理学，免疫组织化学，和炎症因子分析。该实验动物模型为研究囊炎的发病机制和治疗提供了基础。
