Abstract：
Bacillus licheniformis and bacitracin have a huge application market and value in the fields of medicine, chemistry, aquaculture, agricultural, and sideline products. Therefore, the selection of B. licheniformis with high production of bacitracin is of great importance. In this experimental protocol, Bacillus with a high yield of bacitracin was isolated, purified, and identified from the fresh feces of healthy pigs. The inhibitory effect of secondary metabolite bacitracin on Micrococcus luteus was also tested. Thin-layer chromatography and high-performance liquid chromatography were used for the qualitative and quantitative detection of bacitracin. The physiological and biochemical characteristics of B. licheniformis were determined by relevant kits. The phylogenetic relationships of B. licheniformis were determined and constructed using gene sequence detection. This protocol describes and introduces the standard isolation, purification, and identification process of B. licheniformis from animal fresh feces from multiple perspectives, providing a method for the large-scale utilization of B. licheniformis and bacitracin in factories.
摘要：
地衣芽孢杆菌和杆菌肽在医药领域有着巨大的应用市场和应用价值,化学,水产养殖,农业,和副产品。因此,选择具有高产量杆菌肽的地衣芽孢杆菌具有重要意义。在这个实验方案中，分离出高产量的杆菌肽，纯化,并从健康猪的新鲜粪便中鉴定出来。还测试了次级代谢产物杆菌肽对藤黄微球菌的抑制作用。采用薄层色谱法和高效液相色谱法对杆菌肽进行定性和定量检测。通过相关试剂盒测定地衣芽孢杆菌的生理生化特性。通过基因序列检测，确定并构建了地衣芽孢杆菌的系统发育关系。该协议描述并介绍了标准隔离，净化,从多个角度对动物新鲜粪便中地衣芽孢杆菌的鉴定过程，为地衣芽孢杆菌和杆菌肽在工厂的大规模利用提供了方法。
