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Abstract：
This paper provides a complete protocol for studying the effects of inhaled paraquat (PQ), a toxic herbicide that has negative effects systemically and on the lungs. The protocol aims to evaluate the effects of aerosolized PQ exposure on lung and systemic injury in an animal model, which will provide significant information for therapeutic interventions for PQ-induced pulmonary and systemic damage. The protocol involves the following key components: 1. Study groups: By including control, non-treated aerosolized PQ-exposed, and treated PQ-exposed animals with various agent groups in the experiment, lung and systemic injury in each group could be evaluated, and different measured parameters could be compared among groups. 2. PQ exposure: Animals in the PQ-exposed groups are subjected to PQ aerosol inhalation, simulating occupational or accidental exposure in farmers working with this herbicide. 3. Assessment measures: To determine the degree of lung and systemic injury and its physiological effects, several assessments, such as biochemical markers, histopathological analysis, and functional tests, are used. The protocol offers reliable and accurate results by using standardized methods and data collection. The effect of PQ exposure on lung and systemic injury could be evaluated by statistical analysis of the collected data, which also makes it easier to identify possible protective agents or interventions. This comprehensive evaluation protocol provides an essential basis for studying the mechanisms behind PQ-induced lung and systemic injury and assessing the effectiveness of preventative or therapeutic strategies in minimizing its adverse effects.
摘要：
本文提供了一个完整的协议，用于研究吸入百草枯（PQ）的影响，一种对全身和肺部有负面影响的有毒除草剂。该方案旨在评估雾化PQ暴露对动物模型中肺和全身损伤的影响，这将为PQ引起的肺和全身损伤的治疗干预提供重要信息。该协议涉及以下关键组件:1.研究组：通过包括对照，未经处理的雾化PQ暴露，并在实验中用各种试剂组处理PQ暴露的动物，可以评估每组的肺和全身损伤，并且可以在组间比较不同的测量参数。2.PQ暴露：PQ暴露组中的动物接受PQ雾化吸入，模拟使用这种除草剂的农民的职业或意外暴露。3.评估措施：确定肺和全身损伤的程度及其生理效应，几项评估，如生化标志物，组织病理学分析,和功能测试，使用。该协议通过使用标准化方法和数据收集提供可靠和准确的结果。PQ暴露对肺和全身损伤的影响可以通过对收集的数据进行统计分析来评估，这也使得更容易识别可能的保护剂或干预措施。这种全面的评估方案为研究PQ诱导的肺和全身损伤背后的机制以及评估预防或治疗策略在最小化其不良反应方面的有效性提供了必要的基础。
