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Abstract：
UNASSIGNED: The current study aimed to assess the frequency of CDH1 promoter gene hypermethylation in gastric cancer and chronic gastritis and its correlation with clinicopathological aspects.
UNASSIGNED: Methylation-specific PCR was used to detect CDH1 promoter gene hypermethylation in 53 chronic gastritis patients and 40 gastric cancer patients along with normal adjacent tissues.
UNASSIGNED: The chronic gastritis group comprised 29 males and 24 females with a mean age of 51.8 ± 12.96 years, and 49.1 % of them were positive for H. pylori infection. The frequency of CDH1 hypermethylation in gastritis lesions was 18.8 %. CDH1 hypermethylation showed a significant correlation with H. pylori infection (p = 0.039), but no significant association was observed with other clinical features. The gastric cancer group consisted of individuals with a mean age of 65.4 ± 10.6, among them, 77.5 % were male and 22.5 % were female, 62.5 % had PT3 tumors, 40 % had PN1 lymph node involvement, and the majority (47.5 %) of samples were obtained from body segment. CDH1 hypermethylation was significantly associated with depth of invasion (p = 0.017) and nodal invasion (p = 0.041) in this group. In both groups, normal adjacent specimens lacked CDH1 hypermethylation, and there was no statistically significant correlation between CDH1 hypermethylation and age at which the tumor was diagnosed, gender, activity level, or tumor location.
UNASSIGNED: This study demonstrates that E-cadherin methylation is associated with some characteristics of chronic gastritis and gastric cancer. These findings support previous research indicating that CDH1 hypermethylation may play a significant role in the development of gastric cancer.
UNASSIGNED: Methylation-specific PCR was used to detect CDH1 promoter gene hypermethylation in 53 chronic gastritis patients and 40 gastric cancer patients along with normal adjacent tissues.
UNASSIGNED: The chronic gastritis group comprised 29 males and 24 females with a mean age of 51.8 ± 12.96 years, and 49.1 % of them were positive for H. pylori infection. The frequency of CDH1 hypermethylation in gastritis lesions was 18.8 %. CDH1 hypermethylation showed a significant correlation with H. pylori infection (p = 0.039), but no significant association was observed with other clinical features. The gastric cancer group consisted of individuals with a mean age of 65.4 ± 10.6, among them, 77.5 % were male and 22.5 % were female, 62.5 % had PT3 tumors, 40 % had PN1 lymph node involvement, and the majority (47.5 %) of samples were obtained from body segment. CDH1 hypermethylation was significantly associated with depth of invasion (p = 0.017) and nodal invasion (p = 0.041) in this group. In both groups, normal adjacent specimens lacked CDH1 hypermethylation, and there was no statistically significant correlation between CDH1 hypermethylation and age at which the tumor was diagnosed, gender, activity level, or tumor location.
UNASSIGNED: This study demonstrates that E-cadherin methylation is associated with some characteristics of chronic gastritis and gastric cancer. These findings support previous research indicating that CDH1 hypermethylation may play a significant role in the development of gastric cancer.
摘要：
■本研究旨在评估胃癌和慢性胃炎中CDH1启动子基因高甲基化的频率及其与临床病理方面的相关性。
■甲基化特异性PCR检测了53例慢性胃炎患者和40例胃癌患者以及正常癌旁组织的CDH1启动子基因甲基化。
■慢性胃炎组包括29名男性和24名女性，平均年龄为51.8±12.96岁，其中49.1%为幽门螺杆菌感染阳性。胃炎皮损中CDH1甲基化的频率为18.8%。CDH1甲基化与H.pylori感染呈显著正相关(p=0.039),但未观察到与其他临床特征的显著关联.胃癌组由平均年龄为65.4±10.6的个体组成，其中，77.5%为男性，22.5%为女性，62.5%有PT3肿瘤，40%有PN1淋巴结受累,大部分(47.5%)样本来自身体节段。CDH1甲基化与该组的浸润深度（p=0.017）和淋巴结浸润（p=0.041）显着相关。在这两组中,正常相邻标本缺乏CDH1超甲基化，CDH1甲基化与肿瘤诊断年龄之间无统计学意义的相关性,性别,活动水平,或肿瘤位置。
■本研究表明E-cadherin甲基化与慢性胃炎和胃癌的某些特征相关。这些发现支持了先前的研究，表明CDH1甲基化可能在胃癌的发展中起重要作用。
■甲基化特异性PCR检测了53例慢性胃炎患者和40例胃癌患者以及正常癌旁组织的CDH1启动子基因甲基化。
■慢性胃炎组包括29名男性和24名女性，平均年龄为51.8±12.96岁，其中49.1%为幽门螺杆菌感染阳性。胃炎皮损中CDH1甲基化的频率为18.8%。CDH1甲基化与H.pylori感染呈显著正相关(p=0.039),但未观察到与其他临床特征的显著关联.胃癌组由平均年龄为65.4±10.6的个体组成，其中，77.5%为男性，22.5%为女性，62.5%有PT3肿瘤，40%有PN1淋巴结受累,大部分(47.5%)样本来自身体节段。CDH1甲基化与该组的浸润深度（p=0.017）和淋巴结浸润（p=0.041）显着相关。在这两组中,正常相邻标本缺乏CDH1超甲基化，CDH1甲基化与肿瘤诊断年龄之间无统计学意义的相关性,性别,活动水平,或肿瘤位置。
■本研究表明E-cadherin甲基化与慢性胃炎和胃癌的某些特征相关。这些发现支持了先前的研究，表明CDH1甲基化可能在胃癌的发展中起重要作用。
