PDF(Pubmed)
Abstract：
Healing of chronic diabetic wounds is challenging due to complications of severe inflammatory microenvironment, bacterial infection and poor vascular formation. Herein, a novel injectable polyvinyl alcohol-hyaluronic acid-based composite hydrogel was developed, with tannic acid (TA) and silicate functionalization to fabricate an \'all-in-one\' hydrogel PTKH. On one hand, after being locally injected into the wound site, the hydrogel underwent a gradual sol-gel transition in situ, forming an adhesive and protective dressing for the wound. Manipulations of rheological characteristics, mechanical properties and swelling ability of PTKH could be performed via regulating TA and silicate content in hydrogel. On the other hand, PTKH was capable of eliminating reactive oxygen species overexpression, combating infection and generating a cell-favored microenvironment for wound healing acceleration in vitro. Subsequent animal studies demonstrated that PTKH could greatly stimulate angiogenesis and epithelization, accompanied with inflammation and infection risk reduction. Therefore, in consideration of its impressive in vitro and in vivo outcomes, this \'all-in-one\' multifunctional hydrogel may hold promise for chronic diabetic wound treatment.
摘要：
由于严重炎症微环境的并发症，慢性糖尿病伤口的愈合具有挑战性。细菌感染和血管形成不良。在这里，开发了一种新型的可注射聚乙烯醇-透明质酸基复合水凝胶，用单宁酸(TA)和硅酸盐官能化制造“多合一”水凝胶PTKH。一方面,在局部注射到伤口部位后，水凝胶在原位经历了逐渐的溶胶-凝胶转变，形成伤口的粘性和保护性敷料。流变特性的操纵，PTKH的力学性能和溶胀能力可以通过调节水凝胶中的TA和硅酸盐含量来实现。另一方面,PTKH能够消除活性氧的过度表达，在体外对抗感染并产生细胞有利于伤口愈合的微环境。随后的动物研究表明，PTKH可以极大地刺激血管生成和上皮形成，伴随炎症和感染风险降低。因此,考虑到其令人印象深刻的体外和体内结果，这种“多合一”多功能水凝胶可能有望用于慢性糖尿病伤口治疗。
