PDF(Pubmed)
Abstract：
BACKGROUND: The accumulation of oxidized LDL (ox-LDL) in macrophages in association with platelet activity leads to the formation of foam cells, which play a key role in the pathophysiology of atherosclerosis and coronary artery diseases (CAD). Here, in this study, we aimed to investigate the simultaneous effect of ox-LDL and platelets on foam cell formation, as well as modification in cell markers.
METHODS: First, the U937, a human monocytic cell line, was cultured in RPMI-1640. Then, isolated platelets were co-cultured with the U937 and exposed to ox-LDL (80 µg/ml) to evaluate the impact of ox-LDL on foam cell formation using Oil red O (ORO) staining. Also, the expression of foam cells\' surface markers and CD36, ABCA1, SR-B1, ACAT1, and LXRα genes, which are involved in macrophage metabolism and ox-LDL uptake, was measured by flow cytometry and real-time PCR, respectively.
RESULTS: Our findings suggest that platelets promoted foam cell formation (ORO-positive cells), accompanied by a higher level of CD163+ M2 macrophages. Furthermore, the expression of CD36, ABCA1, SR-B1, ACAT1, and LXRα genes, which are implicated in cholesterol accumulation in macrophages, was significantly upregulated in the ox-LDL+ platelets group compared to the control (P < 0.05). Moreover, the up-regulation of CD36, ABCA1, and SR-B1 genes in the ox-LDL+ platelets group was more accentuated compared to the ox-LDL group (P < 0.05).
CONCLUSIONS: Owing to the positive effector role of platelets in the formation of foam cells and CD163+ cells, it could be assumed that platelets play a dual role in the development of these cells.
METHODS: First, the U937, a human monocytic cell line, was cultured in RPMI-1640. Then, isolated platelets were co-cultured with the U937 and exposed to ox-LDL (80 µg/ml) to evaluate the impact of ox-LDL on foam cell formation using Oil red O (ORO) staining. Also, the expression of foam cells\' surface markers and CD36, ABCA1, SR-B1, ACAT1, and LXRα genes, which are involved in macrophage metabolism and ox-LDL uptake, was measured by flow cytometry and real-time PCR, respectively.
RESULTS: Our findings suggest that platelets promoted foam cell formation (ORO-positive cells), accompanied by a higher level of CD163+ M2 macrophages. Furthermore, the expression of CD36, ABCA1, SR-B1, ACAT1, and LXRα genes, which are implicated in cholesterol accumulation in macrophages, was significantly upregulated in the ox-LDL+ platelets group compared to the control (P < 0.05). Moreover, the up-regulation of CD36, ABCA1, and SR-B1 genes in the ox-LDL+ platelets group was more accentuated compared to the ox-LDL group (P < 0.05).
CONCLUSIONS: Owing to the positive effector role of platelets in the formation of foam cells and CD163+ cells, it could be assumed that platelets play a dual role in the development of these cells.
摘要：
背景：与血小板活性相关的巨噬细胞中氧化LDL（ox-LDL）的积累导致泡沫细胞的形成，在动脉粥样硬化和冠状动脉疾病（CAD）的病理生理学中起关键作用。这里,在这项研究中，我们的目的是研究ox-LDL和血小板对泡沫细胞形成的同时作用，以及细胞标记的修饰。
方法：首先，U937，一种人类单核细胞系，在RPMI-1640中培养。然后，分离的血小板与U937共培养并暴露于ox-LDL(80µg/ml),以使用油红O(ORO)染色评估ox-LDL对泡沫细胞形成的影响.此外，泡沫细胞表面标记和CD36、ABCA1、SR-B1、ACAT1和LXRα基因的表达，参与巨噬细胞代谢和ox-LDL摄取，通过流式细胞术和实时PCR检测，分别。
结果：我们的研究结果表明，血小板促进了泡沫细胞的形成（ORO阳性细胞），伴有较高水平的CD163+M2巨噬细胞。此外，CD36，ABCA1，SR-B1，ACAT1和LXRα基因的表达，这与巨噬细胞中胆固醇的积累有关，与对照组相比，ox-LDL+血小板组明显上调（P<0.05）。此外，与ox-LDL组相比,ox-LDL+血小板组CD36、ABCA1和SR-B1基因的上调更为明显(P<0.05)。
结论：由于血小板在泡沫细胞和CD163+细胞形成中的积极效应作用，可以认为血小板在这些细胞的发育中起着双重作用。
方法：首先，U937，一种人类单核细胞系，在RPMI-1640中培养。然后，分离的血小板与U937共培养并暴露于ox-LDL(80µg/ml),以使用油红O(ORO)染色评估ox-LDL对泡沫细胞形成的影响.此外，泡沫细胞表面标记和CD36、ABCA1、SR-B1、ACAT1和LXRα基因的表达，参与巨噬细胞代谢和ox-LDL摄取，通过流式细胞术和实时PCR检测，分别。
结果：我们的研究结果表明，血小板促进了泡沫细胞的形成（ORO阳性细胞），伴有较高水平的CD163+M2巨噬细胞。此外，CD36，ABCA1，SR-B1，ACAT1和LXRα基因的表达，这与巨噬细胞中胆固醇的积累有关，与对照组相比，ox-LDL+血小板组明显上调（P<0.05）。此外，与ox-LDL组相比,ox-LDL+血小板组CD36、ABCA1和SR-B1基因的上调更为明显(P<0.05)。
结论：由于血小板在泡沫细胞和CD163+细胞形成中的积极效应作用，可以认为血小板在这些细胞的发育中起着双重作用。
