PDF(Pubmed)
Abstract：
Tinospora cordifolia, commonly known as \"Giloy\" or \"Guduchi,\" is a medicinal plant with a rich history in traditional medicine systems. The aqueous extract of Tinospora cordifolia stems has garnered attention due to its reported pharmacological activities. This study aimed to investigate the in vitro biological properties of the aqueous extract and complement the findings with in silico studies to gain insights into potential molecular interactions. The Tinospora cordifolia stem aqueous extract was subjected to a battery of in vitro assays to assess its biological properties. Anti-inflammatory activity was evaluated using invitro assay. To complement the in vitro findings, in silico studies involving molecular docking analyses were conducted to predict potential interactions between the extract\'s constituents and relevant biomolecular targets. The in vitro evaluation revealed significant anti-inflammatory activity of the Tinospora cordifolia stem aqueous extract, as evidenced by its ability to suppress the production of pro-inflammatory cytokines. In silico studies provided insights into the molecular interactions between the extract\'s bioactive constituents and key inflammatory and antioxidant targets, further supporting the observed biological properties. The combined in vitro biological assays and in silico studies offer a comprehensive assessment of the Tinospora cordifolia stem aqueous extract\'s potential therapeutic properties. The demonstrated anti-inflammatory activities align with the traditional use of Tinospora cordifolia and suggest its potential in managing inflammatory and oxidative stress-related disorders. The in silico insights provide a molecular understanding of the extract\'s mode of action, strengthening the rationale for further investigation and development of natural products derived from Tinospora cordifolia for pharmaceutical and medicinal applications.
摘要：
山孢,通常被称为“Giloy”或“Guduchi”，“是一种在传统医学系统中具有丰富历史的药用植物。Tinospora茎的水提取物由于其已报道的药理活性而引起了人们的关注。本研究旨在研究水提取物的体外生物学特性，并通过计算机研究补充研究结果，以深入了解潜在的分子相互作用。对Tinospora茎水提取物进行了一系列体外测定，以评估其生物学特性。使用体外试验评价抗炎活性。为了补充体外研究结果，进行了涉及分子对接分析的计算机研究，以预测提取物成分与相关生物分子靶标之间的潜在相互作用。体外评估显示了Tinosporacerdifolia茎水提取物的显着抗炎活性，其抑制促炎细胞因子产生的能力证明了这一点。计算机模拟研究提供了对提取物的生物活性成分与关键炎症和抗氧化剂靶标之间的分子相互作用的见解，进一步支持观察到的生物学特性。联合的体外生物学分析和计算机模拟研究提供了一个全面的评估Tinosporacordifolia茎水提取物的潜在治疗特性。已证明的抗炎活性与Tinosporacordifolia的传统用途一致，并表明其在治疗炎症和氧化应激相关疾病中的潜力。计算机上的见解提供了对提取物作用模式的分子理解，加强进一步研究和开发来自Tinosporacerdifolia的天然产品的理由，用于药物和医药应用。
