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Abstract：
Fungal effectors play a crucial role in the interaction between pathogenic fungi and their hosts. These interactions directly influence the invasion and spread of pathogens, and the development of diseases. Common in fungal extracellular membrane (CFEM) effectors are closely associated with the pathogenicity, cell wall stability, and pathogenic processes of pathogenic fungi. The aim of this study was to investigate the role of CFEM proteins in Neostagonosporella sichuanensis in pathogen-host interactions. We retrieved 19 proteins containing CFEM structural domains from the genome of N. sichuanensis. By systematic analysis, five NsCFEM proteins had signal peptides but lacked transmembrane structural domains, and thus were considered as potential effectors. Among them, NsCFEM1 and NsCFEM2 were successfully cloned and their functions were further investigated. The validation results show that NsCFEM1 was localized in the cell membrane and nucleus, whereas NsCFEM2 was exclusively observed in the cell membrane. Both were identified as secreted proteins. Additionally, NsCFEM1 inhibited Bax-induced programmed cell death in Nicotiana benthamiana, whereas NsCFEM2 did not induce or inhibit this response. NsCFEM1 was implicated as a virulence factor that contributes to fungal growth, development, stress response, and pathogenicity. NsCFEM2 was implicated in maintenance of cell wall stability. This study lays a foundation for elucidating the role of CFEM proteins in the pathogen of fishscale bamboo rhombic-spot caused by N. sichuanensis. In particular, the functional studies of NsCFEM1 and NsCFEM2 revealed their potential roles in the interaction between N. sichuanensis and the host Phyllostachys heteroclada.
摘要：
真菌效应在病原真菌与其宿主的相互作用中起着至关重要的作用。这些相互作用直接影响病原体的侵袭和传播,和疾病的发展。常见的真菌细胞外膜(CFEM)效应子与致病性密切相关,细胞壁稳定性，和病原真菌的致病过程。这项研究的目的是研究CFEM蛋白在病原体-宿主相互作用中在四川新风球藻中的作用。我们从四川N.基因组中检索到19种含有CFEM结构域的蛋白质。通过系统分析,五种NsCFEM蛋白具有信号肽，但缺乏跨膜结构域，因此被认为是潜在的影响因素。其中,成功克隆了NsCFEM1和NsCFEM2，并对其功能进行了进一步研究。验证结果表明,NsCFEM1定位于细胞膜和细胞核,而NsCFEM2仅在细胞膜中观察到。两者都被鉴定为分泌蛋白。此外,NsCFEM1抑制Bax诱导的烟草细胞程序性死亡，而NsCFEM2不诱导或抑制这种反应。NsCFEM1被认为是有助于真菌生长的毒力因子，发展,应激反应，和致病性。NsCFEM2与维持细胞壁稳定性有关。本研究为阐明CFEM蛋白在四川N.chuansis引起的鱼鳞竹菱形病病原体中的作用奠定了基础。特别是,NsCFEM1和NsCFEM2的功能研究揭示了它们在四川N.与寄主毛竹之间的相互作用中的潜在作用。
