PDF(Pubmed)
Abstract：
Sunset Yellow (SY), a synthetic food dye, is widely used in the food industry worldwide. The acceptable daily dosage for SY is 2.5 mg/kg/bw in humans. If SY is consumed in overdosage, it may cause histopathological effects in several organs. Studies in the literature about the effects of SY on growth and development in mammals are contradictory, and there are not enough of them. The investigation aims to determine SY\'s effects on the stomach and small intestine in different age groups of mice using histological methods. Control and treatment groups were created via mice aged 4, 8, and 10 weeks (n = 6). SY was administered by gavage at a level of 30 mg/kg/bw for 28 days to treatment groups. On the last day of the study, the mice were weighed and sacrificed by cervical dislocation. Stomach and small intestine tissues were removed from mice and transferred to 10 % formaldehyde. After passing through alcohol and xylene series and staining with Hematoxylin-Eosin, the tissues were evaluated under light and electron microscopy. The mean body weight (p = 0.01), mean stomach weight (p = 0.03), and mean small intestine weight were increased (p = 0.02) in treatment groups. In these groups, ruptures, fractures, and hemorrhage were detected in the small intestine tissue. In the stomach tissue, necrotic areas and hemorrhage were detected among the epithelial cells. The degenerations were more advanced in the weaning group. SY may be more harmful during weaning and puberty, but additional long-term studies are needed on the subject.
摘要：
日落黄（SY），一种合成的食用染料，广泛应用于世界范围内的食品工业。在人类中，SY的可接受日剂量为2.5mg/kg/bw。如果SY过量食用，它可能在几个器官中引起组织病理学影响。文献中关于SY对哺乳动物生长发育影响的研究是相互矛盾的,他们还不够。本研究旨在利用组织学方法确定SY对不同年龄组小鼠胃和小肠的影响。通过4、8和10周龄的小鼠创建对照组和治疗组(n=6)。SY以30mg/kg/bw的水平通过管饲法给予治疗组28天。在研究的最后一天，将小鼠称重并通过颈椎脱位处死。从小鼠中取出胃和小肠组织并转移到10%甲醛中。通过酒精和二甲苯系列并用苏木精-伊红染色后，在光学和电子显微镜下评估组织。平均体重（p=0.01），平均胃重（p=0.03），治疗组平均小肠重量增加(p=0.02)。在这些群体中,破裂,骨折,在小肠组织中检测到出血。在胃组织中，在上皮细胞中检测到坏死区域和出血。断奶组的退化更为先进。SY在断奶和青春期可能更有害，但需要对该主题进行更多的长期研究。
