PDF(Pubmed)
Abstract：
Bioluminescence is light chemically produced by an organism. It is widespread across all major marine phyla and has evolved multiple times, resulting in a high diversity of spectral properties and first flash kinetic parameters (FFKP). The bioluminescence of a system is often a good proxy for planktonic biomass. The species-specific parameters of bioluminescent displays can be measured to identify species in situ and describe planktonic biodiversity. Most bioluminescent organisms will flash when mechanically stimulated i.e., when subjected to supra-threshold levels of shear stress. Here we compare first flash kinetic parameters such as flash duration, peak intensity, rise time, decay time, first-flash mechanically stimulated light and e-folding time obtained with the commercially available Underwater Bioluminescence Assessment Tool (UBAT). We provide descriptions of the first flash kinetic parameters of several species of dinoflagellates Pyrocystis fusiformis, Pyrocystis noctiluca, Pyrodinium bahamense, Lingulodinium polyedra, Alexandrium monilatum and two zooplankton (the ctenophore Mnemiopsis leidyi and the larvacean Oikopleura sp.). FFKPs are then compared and discussed using non-parametric analyses of variance (ANOVAs), hierarchical clustering and a linear discriminant analysis to assess the ability to use bioluminescence signatures for identification. Once the first flash kinetic parameters of a bioluminescent species have been described, it is possible to detect its presence using emissions collected by in situ bathyphotometers. Assessing abundance and diversity of bioluminescent species may therefore be possible.
摘要：
生物发光是由生物体化学产生的光。它在所有主要的海洋门都很普遍，并且已经进化了多次，导致光谱特性和第一闪蒸动力学参数(FFKP)的高度多样性。系统的生物发光通常是浮游生物量的良好代表。可以测量生物发光显示器的物种特定参数，以原位识别物种并描述浮游生物多样性。大多数生物发光生物在受到机械刺激时会闪烁，即当受到超阈值水平的剪切应力时。在这里，我们比较第一闪光动力学参数，如闪光持续时间，峰值强度,上升时间,衰减时间,使用市售水下生物发光评估工具（UBAT）获得的第一闪光机械刺激光和电子折叠时间。我们提供了几种鞭毛藻的第一个闪光动力学参数的描述，夜光囊虫,pyrodiniumbahamense,柳叶刀，Alexandriummonilatum和两个浮游动物（ctenophoreMnemimopsisleidyi和幼虫Oikopleurasp。).然后使用非参数方差分析(ANOVA)比较和讨论FFKP，分层聚类和线性判别分析，以评估使用生物发光特征进行识别的能力。一旦描述了生物发光物种的第一个闪光动力学参数，可以使用原位测深光度计收集的排放物来检测其存在。因此,评估生物发光物种的丰度和多样性是可能的。
