PDF(Pubmed)
Abstract：
UNASSIGNED: Cancer has surpassed infectious diseases and heart ailments, taking the top spot in the disease hierarchy. Cervical cancer is a significant concern for women due to high incidence and mortality rates, linked to the human papillomavirus (HPV). HPV infection leads to precancerous lesions progressing to cervical cancer. The cervix\'s external os, near the vagina, hosts various microorganisms. Evidence points to the link between vaginal microbiota and HPV-induced cervical cancer. Cervical cancer onset aligns with an imbalanced Th1/Th2 immune response, but the role of vaginal microbiota in modulating this imbalance is unclear.
UNASSIGNED: In this study, we collected vaginal samples from 99 HPV-infected patients across varying degrees of lesions, alongside control groups. These samples underwent bacterial DNA sequencing. Additionally, we employed Elisa kits to quantify the protein expression levels of Th1/Th2 cytokines IL2, IL12, IL5, IL13, and TNFa within the centrifuged supernatant of vaginal-cervical secretions from diverse research subjects. Subsequently, correlation analyses were conducted between inflammatory factors and vaginal microbiota.
UNASSIGNED: Our findings highlighted a correlation between decreased Lactobacillus and increased Gardenerella presence with HPV-induced cervical cancer. Functionally, our predictive analysis revealed the predominant enrichment of the ABC transporter within the vaginal microbiota of cervical cancer patients. Notably, these microbiota alterations exhibited correlations with the production of Th1/Th2 cytokines, which are intimately tied to tumor immunity.
UNASSIGNED: This study suggests the potential involvement of vaginal microbiota in the progression of HPV-induced cervical cancer through Th1/Th2 cytokine regulation. This novel insight offers a fresh perspective for early cervical cancer diagnosis and future prevention strategies.
UNASSIGNED: In this study, we collected vaginal samples from 99 HPV-infected patients across varying degrees of lesions, alongside control groups. These samples underwent bacterial DNA sequencing. Additionally, we employed Elisa kits to quantify the protein expression levels of Th1/Th2 cytokines IL2, IL12, IL5, IL13, and TNFa within the centrifuged supernatant of vaginal-cervical secretions from diverse research subjects. Subsequently, correlation analyses were conducted between inflammatory factors and vaginal microbiota.
UNASSIGNED: Our findings highlighted a correlation between decreased Lactobacillus and increased Gardenerella presence with HPV-induced cervical cancer. Functionally, our predictive analysis revealed the predominant enrichment of the ABC transporter within the vaginal microbiota of cervical cancer patients. Notably, these microbiota alterations exhibited correlations with the production of Th1/Th2 cytokines, which are intimately tied to tumor immunity.
UNASSIGNED: This study suggests the potential involvement of vaginal microbiota in the progression of HPV-induced cervical cancer through Th1/Th2 cytokine regulation. This novel insight offers a fresh perspective for early cervical cancer diagnosis and future prevention strategies.
摘要：
癌症已经超过传染病和心脏病,在疾病等级中名列前茅。由于宫颈癌的发病率和死亡率高，宫颈癌是女性的重要关注点，与人乳头瘤病毒（HPV）有关。HPV感染导致癌前病变进展为宫颈癌。子宫颈的外部操作系统，在阴道附近,宿主各种微生物。有证据表明阴道微生物群与HPV诱导的宫颈癌之间存在联系。宫颈癌发病与Th1/Th2免疫反应不平衡，但阴道微生物群在调节这种失衡中的作用尚不清楚。
■在这项研究中，我们收集了99例HPV感染患者的不同程度病变的阴道样本，与对照组并驾齐驱。对这些样品进行细菌DNA测序。此外,我们使用Elisa试剂盒定量来自不同研究对象的阴道-宫颈分泌物离心上清液中Th1/Th2细胞因子IL2,IL12,IL5,IL13和TNFa的蛋白表达水平.随后，对炎症因子与阴道菌群进行相关性分析。
■我们的研究结果强调了在HPV诱导的宫颈癌中，乳酸菌的减少和栀子菌的增加之间的相关性。功能上,我们的预测分析显示,宫颈癌患者的阴道微生物群中ABC转运体的主要富集.值得注意的是,这些微生物群改变与Th1/Th2细胞因子的产生相关，与肿瘤免疫密切相关。
■本研究提示阴道微生物群可能通过调节Th1/Th2细胞因子参与HPV诱导的宫颈癌的进展。这种新颖的见解为早期宫颈癌诊断和未来的预防策略提供了新的视角。
■在这项研究中，我们收集了99例HPV感染患者的不同程度病变的阴道样本，与对照组并驾齐驱。对这些样品进行细菌DNA测序。此外,我们使用Elisa试剂盒定量来自不同研究对象的阴道-宫颈分泌物离心上清液中Th1/Th2细胞因子IL2,IL12,IL5,IL13和TNFa的蛋白表达水平.随后，对炎症因子与阴道菌群进行相关性分析。
■我们的研究结果强调了在HPV诱导的宫颈癌中，乳酸菌的减少和栀子菌的增加之间的相关性。功能上,我们的预测分析显示,宫颈癌患者的阴道微生物群中ABC转运体的主要富集.值得注意的是,这些微生物群改变与Th1/Th2细胞因子的产生相关，与肿瘤免疫密切相关。
■本研究提示阴道微生物群可能通过调节Th1/Th2细胞因子参与HPV诱导的宫颈癌的进展。这种新颖的见解为早期宫颈癌诊断和未来的预防策略提供了新的视角。
