PDF(Pubmed)
Abstract：
UNASSIGNED: In recent years, fluoride concentrations in toothpaste for children and adults have increased. However, the effects of different concentrations on bacterial activity have rarely been compared. We aimed to investigate and compare the antibacterial activity of children\'s and adults\' toothpaste containing 500, 1000‒1100, and 1450‒1500 ppm fluoride.
UNASSIGNED: Three strains of bacteria (Streptococcus mutans, Streptococcus salivarius, and Lactobacillus casei) were cultured in brain heart infusion agar. Thirty commercially available toothpaste products for children and adults containing 500, 1000‒1100, and 1450‒1500 ppm fluoride were selected and tested. Toothpaste\'s ability to inhibit bacterial growth was evaluated by agar diffusion assay, in which plates were incubated for 24 hours, and then the diameter of the microbial inhibition zone was measured. Comparisons between children\'s and adults\' fluoride toothpastes were made using the Mann-Whitney U test. The association between bacterial growth inhibition and sodium lauryl sulfate (SLS) was analyzed by the chi-square test. A P value of <0.05 was considered statistically significant.
UNASSIGNED: No difference in the inhibition zone was observed for different fluoride concentrations. However, there were significant differences between toothpastes for children and adults, with higher inhibition zones for adults\' toothpastes. Most toothpastes for adults contained SLS, which was associated with antibacterial activity.
UNASSIGNED: Fluoride concentrations ranging from 500 to 1500 ppm did not affect bacterial growth. The antibacterial activity of toothpastes for adults was significantly higher than that of toothpastes for children, which was mainly attributed to the SLS usually added to adult formulations.
UNASSIGNED: Three strains of bacteria (Streptococcus mutans, Streptococcus salivarius, and Lactobacillus casei) were cultured in brain heart infusion agar. Thirty commercially available toothpaste products for children and adults containing 500, 1000‒1100, and 1450‒1500 ppm fluoride were selected and tested. Toothpaste\'s ability to inhibit bacterial growth was evaluated by agar diffusion assay, in which plates were incubated for 24 hours, and then the diameter of the microbial inhibition zone was measured. Comparisons between children\'s and adults\' fluoride toothpastes were made using the Mann-Whitney U test. The association between bacterial growth inhibition and sodium lauryl sulfate (SLS) was analyzed by the chi-square test. A P value of <0.05 was considered statistically significant.
UNASSIGNED: No difference in the inhibition zone was observed for different fluoride concentrations. However, there were significant differences between toothpastes for children and adults, with higher inhibition zones for adults\' toothpastes. Most toothpastes for adults contained SLS, which was associated with antibacterial activity.
UNASSIGNED: Fluoride concentrations ranging from 500 to 1500 ppm did not affect bacterial growth. The antibacterial activity of toothpastes for adults was significantly higher than that of toothpastes for children, which was mainly attributed to the SLS usually added to adult formulations.
摘要：
■近年来，儿童和成人牙膏中的氟化物浓度有所增加。然而,很少比较不同浓度对细菌活性的影响。我们旨在研究和比较含有500、1000-1100和1450-1500ppm氟化物的儿童和成人牙膏的抗菌活性。
■三株细菌（变形链球菌，唾液链球菌，和干酪乳杆菌）在脑心输注琼脂中培养。选择并测试了30种市售的含500、1000-1100和1450-1500ppm氟化物的儿童和成人牙膏产品。通过琼脂扩散试验评估牙膏抑制细菌生长的能力，其中培养板24小时，然后测量微生物抑制区的直径。使用Mann-WhitneyU检验对儿童和成人氟化物牙膏进行了比较。通过卡方检验分析了细菌生长抑制与十二烷基硫酸钠（SLS）之间的关联。P值<0.05被认为具有统计学意义。
■对于不同的氟化物浓度，没有观察到抑制区的差异。然而,儿童牙膏和成人牙膏之间存在显着差异，对成人牙膏有较高的抑制区。大多数成人牙膏含有SLS，这与抗菌活性有关。
■氟化物浓度范围为500至1500ppm不影响细菌生长。成人牙膏的抗菌活性明显高于儿童牙膏,这主要归因于通常添加到成人配方中的SLS。
■三株细菌（变形链球菌，唾液链球菌，和干酪乳杆菌）在脑心输注琼脂中培养。选择并测试了30种市售的含500、1000-1100和1450-1500ppm氟化物的儿童和成人牙膏产品。通过琼脂扩散试验评估牙膏抑制细菌生长的能力，其中培养板24小时，然后测量微生物抑制区的直径。使用Mann-WhitneyU检验对儿童和成人氟化物牙膏进行了比较。通过卡方检验分析了细菌生长抑制与十二烷基硫酸钠（SLS）之间的关联。P值<0.05被认为具有统计学意义。
■对于不同的氟化物浓度，没有观察到抑制区的差异。然而,儿童牙膏和成人牙膏之间存在显着差异，对成人牙膏有较高的抑制区。大多数成人牙膏含有SLS，这与抗菌活性有关。
■氟化物浓度范围为500至1500ppm不影响细菌生长。成人牙膏的抗菌活性明显高于儿童牙膏,这主要归因于通常添加到成人配方中的SLS。
