PDF(Pubmed)
Abstract：
UNASSIGNED: Putative microglia were recently detected using adaptive optics ophthalmoscopy in healthy eyes. Here we evaluate the use of nonconfocal adaptive optics scanning light ophthalmoscopy (AOSLO) for quantifying the morphology and motility of presumed microglia and other immune cells in eyes with retinal inflammation from uveitis and healthy eyes.
UNASSIGNED: Observational exploratory study.
UNASSIGNED: Twelve participants were imaged, including 8 healthy participants and 4 posterior uveitis patients recruited from the clinic of 1 of the authors (M.H.E.).
UNASSIGNED: The Pittsburgh AOSLO imaging system was used with a custom-designed 7-fiber optical fiber bundle for simultaneous confocal and nonconfocal multioffset detection. The inner retina was imaged at several locations at multiple timepoints in healthy participants and uveitis patients to generate time-lapse images.
UNASSIGNED: Microglia and macrophages were manually segmented from nonconfocal AOSLO images, and their morphological characteristics quantified (including soma size, diameter, and circularity). Cell soma motion was quantified across time for periods of up to 30 minutes and their speeds were calculated by measuring their displacement over time.
UNASSIGNED: A spectrum of cell morphologies was detected in healthy eyes from circular amoeboid cells to elongated cells with visible processes, resembling activated and ramified microglia, respectively. Average soma diameter was 16.1 ± 0.9 μm. Cell movement was slow in healthy eyes (0.02 μm/sec on average), but macrophage-like cells moved rapidly in some uveitis patients (up to 3 μm/sec). In an eye with infectious uveitis, many macrophage-like cells were detected; during treatment their quantity and motility decreased as vision improved.
UNASSIGNED: In vivo adaptive optics ophthalmoscopy offers promise as a potentially powerful tool for detecting and monitoring inflammation and response to treatment at a cellular level in the living eye.
UNASSIGNED: Proprietary or commercial disclosure may be found in the Footnotes and Disclosures at the end of this article.
UNASSIGNED: Observational exploratory study.
UNASSIGNED: Twelve participants were imaged, including 8 healthy participants and 4 posterior uveitis patients recruited from the clinic of 1 of the authors (M.H.E.).
UNASSIGNED: The Pittsburgh AOSLO imaging system was used with a custom-designed 7-fiber optical fiber bundle for simultaneous confocal and nonconfocal multioffset detection. The inner retina was imaged at several locations at multiple timepoints in healthy participants and uveitis patients to generate time-lapse images.
UNASSIGNED: Microglia and macrophages were manually segmented from nonconfocal AOSLO images, and their morphological characteristics quantified (including soma size, diameter, and circularity). Cell soma motion was quantified across time for periods of up to 30 minutes and their speeds were calculated by measuring their displacement over time.
UNASSIGNED: A spectrum of cell morphologies was detected in healthy eyes from circular amoeboid cells to elongated cells with visible processes, resembling activated and ramified microglia, respectively. Average soma diameter was 16.1 ± 0.9 μm. Cell movement was slow in healthy eyes (0.02 μm/sec on average), but macrophage-like cells moved rapidly in some uveitis patients (up to 3 μm/sec). In an eye with infectious uveitis, many macrophage-like cells were detected; during treatment their quantity and motility decreased as vision improved.
UNASSIGNED: In vivo adaptive optics ophthalmoscopy offers promise as a potentially powerful tool for detecting and monitoring inflammation and response to treatment at a cellular level in the living eye.
UNASSIGNED: Proprietary or commercial disclosure may be found in the Footnotes and Disclosures at the end of this article.
摘要：
■最近使用自适应光学检眼镜在健康眼睛中检测到推定的小胶质细胞。在这里，我们评估了非共焦自适应光学扫描光检眼镜（AOSLO）的使用，以量化假定的小胶质细胞和其他免疫细胞的形态和运动性与葡萄膜炎和健康眼睛的视网膜炎症。
■观察性探索性研究。
■对12名参与者进行了成像，包括8名健康参与者和从1名作者（M.H.E.）的诊所招募的4名后葡萄膜炎患者。
■匹兹堡AOSLO成像系统与定制设计的7光纤光纤束一起使用，用于同时进行共焦和非共焦多偏移检测。在健康参与者和葡萄膜炎患者的多个时间点在多个位置对内部视网膜进行成像以生成延时图像。
■从非共焦AOSLO图像中手动分割小胶质细胞和巨噬细胞，和它们的形态特征量化(包括体细胞大小，直径,和循环性)。细胞胞体运动在长达30分钟的时间段内被量化,并且通过测量它们随时间的位移来计算它们的速度。
■在健康的眼睛中检测到从圆形变形虫细胞到具有可见过程的细长细胞的细胞形态谱，类似于活化的和分枝的小胶质细胞，分别。平均体细胞直径为16.1±0.9μm。健康眼睛的细胞运动缓慢（平均0.02μm/sec），但是巨噬细胞样细胞在一些葡萄膜炎患者中快速移动（高达3μm/sec）。在患有感染性葡萄膜炎的眼睛中，检测到许多巨噬细胞样细胞；在治疗期间，随着视力的改善，它们的数量和运动性下降。
■体内自适应光学检眼镜有望成为检测和监测活体眼睛中细胞水平的炎症和治疗反应的潜在强大工具。
■专有或商业披露可在本文末尾的脚注和披露中找到。
■观察性探索性研究。
■对12名参与者进行了成像，包括8名健康参与者和从1名作者（M.H.E.）的诊所招募的4名后葡萄膜炎患者。
■匹兹堡AOSLO成像系统与定制设计的7光纤光纤束一起使用，用于同时进行共焦和非共焦多偏移检测。在健康参与者和葡萄膜炎患者的多个时间点在多个位置对内部视网膜进行成像以生成延时图像。
■从非共焦AOSLO图像中手动分割小胶质细胞和巨噬细胞，和它们的形态特征量化(包括体细胞大小，直径,和循环性)。细胞胞体运动在长达30分钟的时间段内被量化,并且通过测量它们随时间的位移来计算它们的速度。
■在健康的眼睛中检测到从圆形变形虫细胞到具有可见过程的细长细胞的细胞形态谱，类似于活化的和分枝的小胶质细胞，分别。平均体细胞直径为16.1±0.9μm。健康眼睛的细胞运动缓慢（平均0.02μm/sec），但是巨噬细胞样细胞在一些葡萄膜炎患者中快速移动（高达3μm/sec）。在患有感染性葡萄膜炎的眼睛中，检测到许多巨噬细胞样细胞；在治疗期间，随着视力的改善，它们的数量和运动性下降。
■体内自适应光学检眼镜有望成为检测和监测活体眼睛中细胞水平的炎症和治疗反应的潜在强大工具。
■专有或商业披露可在本文末尾的脚注和披露中找到。
