Abstract：
Ischemia/reperfusion (I/R) injury is one of the major causes of cardiovascular disease. Gypenoside A (GP), the main active component of Gynostemma pentaphyllum, alleviates myocardial I/R injury. Circular RNAs (circRNAs) and microRNAs (miRNAs) are involved in the I/R injury. We explored the protective effect of GP on human cardiomyocytes (HCMs) via the circ_0010729/miR-370-3p/RUNX1 axis. Overexpression of circ_0010729 abolished the effects of GP on HMC, such as suppression of apoptosis and increase in cell viability and proliferation. Overexpression of miR-370-3p reversed the effect of circ_0010729 overexpression, resulting in the stimulation of HMC viability and proliferation and inhibition of apoptosis. The knockdown of miR-370-3p suppressed the effects of GP in HCMs. RUNX1 silencing counteracted the effect of miR-370-3p knockdown and maintained GP-induced suppression of apoptosis and stimulation of HMC viability and proliferation. The levels of RUNX1 mRNA and protein were reduced in cells expressing miR-370-3p. In conclusion, this study confirmed that GP alleviated the I/R injury of myocardial cell via the circ_0010729/miR-370-3p/RUNX1 axis.
摘要：
缺血/再灌注(I/R)损伤是心血管疾病的主要原因之一。绞股蓝皂甙A(GP),绞股蓝的主要活性成分，减轻心肌I/R损伤。环状RNA(circularRNAs)和微小RNA(microRNAs)参与I/R损伤。我们通过circ_0010729/miR-370-3p/RUNX1轴探索了GP对人心肌细胞(HCM)的保护作用。circ_0010729的过表达消除了GP对HMC的影响，如抑制细胞凋亡和增加细胞活力和增殖。miR-370-3p的过表达逆转了circ_0010729过表达的作用,从而刺激HMC的活力和增殖并抑制细胞凋亡。miR-370-3p的敲低抑制GP在HCM中的作用。RUNX1沉默抵消了miR-370-3p敲低的作用，并维持GP诱导的凋亡抑制和HMC活力和增殖的刺激。RUNX1mRNA和蛋白质的水平在表达miR-370-3p的细胞中降低。总之,本研究证实GP通过circ_0010729/miR-370-3p/RUNX1轴减轻心肌细胞的I/R损伤。
