Abstract：
Biosynthetic intermediates of siderophore vibrioferrin (VF), O-citryl-L-serine, 2-aminoethyl citrate, and alanine-2-amidoethyl citrate were respectively synthesized as a mixture of stereoisomers. These compounds were used as substrates for enzyme reactions using recombinant PvsA, PvsB, and PvsE proteins as corresponding enzyme equivalents. The results of our study show that each enzyme reacts with a respective substrate and produces VF along the proposed biosynthetic pathway. Furthermore, the results of this study will contribute to the understanding of VF biosynthetic enzymes and may help in the development of antimicrobial drugs by inhibiting siderophore biosynthetic enzymes.
摘要：
铁载体弧铁蛋白（VF）的生物合成中间体，O-柠檬酸-L-丝氨酸，柠檬酸2-氨基乙酯,和丙氨酸-2-氨基乙基柠檬酸酯分别合成为立体异构体的混合物。这些化合物被用作使用重组PvsA的酶反应的底物，PvsB,和PvsE蛋白作为相应的酶等价物。我们的研究结果表明，每种酶与各自的底物反应，并沿所提出的生物合成途径产生VF。此外，这项研究的结果将有助于了解VF生物合成酶，并可能有助于通过抑制铁载体生物合成酶来开发抗菌药物。
