Abstract：
Selenium (Se) is one of the fifteen essential nutrients required by the human body. Mycorrhizal microorganisms play a crucial role in enhancing selenium availability in plants. However, limited research exists on the impact of arbuscular mycorrhizal fungi (AMF) on selenium accumulation and transport in pepper plants. This study employed a pot experiment to investigate the changes in pepper plant growth, selenium accumulation, and transformation following inoculation with AMF and varying concentrations of exogenous selenium. The results indicate that exogenous selenium application in pepper has dual effects. At low concentrations (≤8 mg L⁻1), it promotes growth and nutrient accumulation, whereas high concentrations (>16 mg L⁻1) inhibit these processes. AMF inoculation positively influences selenium accumulation and transport in peppers, significantly increasing yield per plant by 17.89%, vitamin C content by 67.36%, flavonoid content by 43.26%, capsaicin content by 14.82%, DPPH radical scavenging rate by 18.18%, and ABTS radical scavenging rate by 27.81%. Additionally, it significantly reduces selenocysteine methyltransferase (SMT) enzyme activity, while minimally affecting ATP sulfurylase (ATPS) and adenosyl sulfate reductase (APR) enzyme activities. The combined treatment of AMF and 8 mg L⁻1 exogenous selenium has been proven to be the most effective for selenium enrichment in peppers, offering new insights into utilizing exogenous selenium and AMF inoculation to enhance selenium content in peppers.
摘要：
硒(Se)是人体必需的十五种营养素之一。菌根微生物在提高植物中硒的利用率中起着至关重要的作用。然而,关于丛枝菌根真菌（AMF）对辣椒植物中硒积累和运输的影响的研究有限。这项研究采用盆栽实验来研究辣椒植物生长的变化，硒的积累，接种AMF和不同浓度的外源硒后进行转化。结果表明,外源施硒对辣椒具有双重效应。在低浓度(≤8毫克升1)时，它促进生长和营养积累，而高浓度(>16毫克升1)抑制这些过程。AMF接种对辣椒中硒的积累和运输有积极影响，单株产量显著提高17.89%，维生素C含量为67.36%,黄酮含量为43.26%,辣椒素含量为14.82%，DPPH自由基清除率达18.18%,ABTS自由基清除率为27.81%。此外,它显著降低硒代半胱氨酸甲基转移酶（SMT）酶活性，同时最低限度地影响ATP硫酸化酶（ATPS）和腺苷硫酸还原酶（APR）的活性。AMF和8mgL→1外源硒的联合处理已被证明是最有效的辣椒富硒，提供了利用外源硒和AMF接种来提高辣椒中硒含量的新见解。
