Abstract：
Intrafollicular Transfer of Immature Oocytes (IFIOT) has emerged as an alternative to the currently used systems for bovine embryo production. This technique associates the rapid multiplication of bovine females under a completely in vivo culture condition, eliminating the need for superstimulatory hormones in the in vivo system (IVD) and the costly laboratory setup required for in vitro embryo production (IVP). Despite being a promising technique, the results obtained to date have been unsatisfactory for commercial use. Only approximately 10 % -12 % of viable embryos are recovered from the total number of injected oocytes, which limits their use in genetic improvement programs. IFIOT problems can occur in any of the steps involved; therefore, each step must be carefully examined to identify those that have the most negative impact on the final embryo recovery. This review summarizes the different studies conducted using the IFIOT to provide a comprehensive analysis of the main factors that can influence the effectiveness of this technique.
摘要：
未成熟卵母细胞的卵泡内转移（IFIOT）已成为当前使用的牛胚胎生产系统的替代方法。该技术将牛雌性在完全体内培养条件下的快速繁殖相关联，消除了体内系统（IVD）中对超刺激激素的需求，以及体外胚胎生产（IVP）所需的昂贵的实验室设备。尽管是一种很有前途的技术，迄今为止获得的结果对于商业用途来说并不令人满意。从注射的卵母细胞总数中只有大约10％-12％的存活胚胎被回收，这限制了它们在基因改良计划中的使用。IFIOT问题可能发生在所涉及的任何步骤中；因此，每个步骤都必须仔细检查，以确定对最终胚胎恢复产生最大负面影响的步骤。这篇综述总结了使用IFIOT进行的不同研究，以提供对可能影响该技术有效性的主要因素的全面分析。
