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Abstract：
BACKGROUND: Piroplasmosis is a common and prevalent tick-borne disease that affects equids.
OBJECTIVE: To determine the infection and molecular characteristics of the piroplasms in donkeys from Xinjiang, northwestern China, we undertook a cross sectional study by collecting representative samples across several counties within the region.
METHODS: A total of 344 blood samples were collected from adult domestic donkeys from 13 counties in Xinjiang. PCR was conducted to test for T. equi and B. caballi in the blood samples based on the equine merozoite antigen-1 (Ema-1) gene and the 48 kDa rhoptry protein (BC48) gene, respectively.
RESULTS: Sixteen blood samples tested positive for piroplasms and the overall infection rate was 4.7% (16/344). Seven of the 13 counties were positive for piroplasms. Among the 16 piroplasm-positive samples, 15 were singly infected with T. equi with an infection rate of 4.4% (15/344), and coinfection with T. equi and B. caballi was detected in one sample (0.3%, 1/344) from Wushi. Four T. equi sequence genotypes were identified and grouped into different branches of the evolutionary trees.
CONCLUSIONS: These findings suggest that the infection rate of piroplasms is low in domestic donkeys in southern Xinjiang and that T. equi genotypes have a regional distribution.
OBJECTIVE: To determine the infection and molecular characteristics of the piroplasms in donkeys from Xinjiang, northwestern China, we undertook a cross sectional study by collecting representative samples across several counties within the region.
METHODS: A total of 344 blood samples were collected from adult domestic donkeys from 13 counties in Xinjiang. PCR was conducted to test for T. equi and B. caballi in the blood samples based on the equine merozoite antigen-1 (Ema-1) gene and the 48 kDa rhoptry protein (BC48) gene, respectively.
RESULTS: Sixteen blood samples tested positive for piroplasms and the overall infection rate was 4.7% (16/344). Seven of the 13 counties were positive for piroplasms. Among the 16 piroplasm-positive samples, 15 were singly infected with T. equi with an infection rate of 4.4% (15/344), and coinfection with T. equi and B. caballi was detected in one sample (0.3%, 1/344) from Wushi. Four T. equi sequence genotypes were identified and grouped into different branches of the evolutionary trees.
CONCLUSIONS: These findings suggest that the infection rate of piroplasms is low in domestic donkeys in southern Xinjiang and that T. equi genotypes have a regional distribution.
摘要：
背景：Piroplasmosis是一种常见且流行的蜱传疾病，会影响马。
目的:了解新疆毛驴丝质的感染情况及分子学特征,中国西北部,我们通过收集该地区几个县的代表性样本进行了横断面研究。
方法：从新疆13个县的成年驴中采集344份血液样本。根据马裂殖子抗原-1（Ema-1）基因和48kDarhoptry蛋白（BC48）基因，进行PCR以测试血液样品中的T.equi和B.caballi，分别。
结果：16份血液样本检测为pirosoma阳性，总感染率为4.7%（16/344）。13个县中有7个为po质阳性。在16份吡罗质阳性样本中，15例单独感染马氏T.equi，感染率为4.4%(15/344)，在一个样本中检测到与T.equi和B.caballi的合并感染(0.3%，1/344）来自乌什。鉴定了四种T.equi序列基因型，并将其分为进化树的不同分支。
结论：这些发现表明，新疆南部的家驴中的丝质感染率较低，并且T.equi基因型具有区域性分布。
目的:了解新疆毛驴丝质的感染情况及分子学特征,中国西北部,我们通过收集该地区几个县的代表性样本进行了横断面研究。
方法：从新疆13个县的成年驴中采集344份血液样本。根据马裂殖子抗原-1（Ema-1）基因和48kDarhoptry蛋白（BC48）基因，进行PCR以测试血液样品中的T.equi和B.caballi，分别。
结果：16份血液样本检测为pirosoma阳性，总感染率为4.7%（16/344）。13个县中有7个为po质阳性。在16份吡罗质阳性样本中，15例单独感染马氏T.equi，感染率为4.4%(15/344)，在一个样本中检测到与T.equi和B.caballi的合并感染(0.3%，1/344）来自乌什。鉴定了四种T.equi序列基因型，并将其分为进化树的不同分支。
结论：这些发现表明，新疆南部的家驴中的丝质感染率较低，并且T.equi基因型具有区域性分布。
