Abstract：
CONCLUSIONS: Ectopic expression of OsWOX9A induces narrow adaxially rolled rice leaves with larger bulliform cells and fewer large veins, probably through regulating the expression of auxin-related and expansin genes. The WUSCHEL-related homeobox (WOX) family plays a pivotal role in plant development by regulating genes involved in various aspects of growth and differentiation. OsWOX9A (DWT1) has been linked to tiller growth, uniform plant growth, and flower meristem activity. However, its impact on leaf growth and development in rice has not been studied. In this study, we investigated the biological role of OsWOX9A in rice growth and development using transgenic plants. Overexpression of OsWOX9A conferred narrow adaxially rolled rice leaves and altered plant architecture. These plants exhibited larger bulliform cells and fewer larger veins compared to wild-type plants. OsWOX9A overexpression also reduced plant height, tiller number, and seed-setting rate. Comparative transcriptome analysis revealed several differentially expressed auxin-related and expansin genes in OsWOX9A overexpressing plants, consistent with their roles in leaf and plant development. These results indicate that the ectopic expression of OsWOX9A may have multiple effects on the development and growth of rice, providing a more comprehensive picture of how the WOX9 subfamily contributes to leaf development and plant architecture.
摘要：
结论：OsWOX9A的异位表达诱导狭窄的正面滚动水稻叶片，具有较大的球形细胞和较少的大静脉，可能通过调节生长素相关和expansin基因的表达。WUSCHEL相关同源异型盒（WOX）家族通过调节涉及生长和分化各个方面的基因，在植物发育中发挥关键作用。OsWOX9A（DWT1）与分till生长有关，植物生长均匀，和花分生组织活动。然而,其对水稻叶片生长发育的影响尚未研究。在这项研究中,我们使用转基因植物研究了OsWOX9A在水稻生长发育中的生物学作用。OsWOX9A的过度表达赋予了狭窄的正面滚动水稻叶片并改变了植物结构。与野生型植物相比，这些植物表现出较大的球形细胞和较少的较大静脉。OsWOX9A过表达也降低了株高，舵柄编号,和结实率。比较转录组分析揭示了OsWOX9A过表达植物中几个差异表达的生长素相关和expansin基因，与它们在叶片和植物发育中的作用一致。这些结果表明，OsWOX9A的异位表达可能对水稻的发育和生长有多重影响。更全面地了解WOX9亚科如何促进叶片发育和植物结构。
