PDF(Pubmed)
Abstract：
BACKGROUND: AP2/ERF is a large family of plant transcription factor proteins that play essential roles in signal transduction, plant growth and development, and responses to various stresses. The AP2/ERF family has been identified and verified by functional analysis in various plants, but so far there has been no comprehensive study of these factors in Chinese prickly ash. Phylogenetic, motif, and functional analyses combined with transcriptome analysis of Chinese prickly ash fruits at different developmental stages (30, 60, and 90 days after anthesis) were conducted in this study.
RESULTS: The analysis identified 146 ZbAP2/ERF genes that could be classified into 15 subgroups. The motif analysis revealed the presence of different motifs or elements in each group that may explain the functional differences between the groups. ZbERF13.2, ZbRAP2-12, and ZbERF2.1 showed high levels of expression in the early stages of fruit development. ZbRAP2-4, and ZbERF3.1 were significantly expressed at the fruit coloring stage (R2 and G2). ZbERF16 were significantly expressed at fruit ripening and expression level increased as the fruit continued to develop. Relative gene expression levels of 6 representative ZbAP2/ERFs assessed by RT-qPCR agreed with transcriptome analysis results.
CONCLUSIONS: These genes identified by screening can be used as candidate genes that affect fruit development. The results of the analysis can help guide future genetic improvement of Chinese prickly ash and enrich our understanding of AP2/ERF transcription factors and their regulatory functions in plants.
RESULTS: The analysis identified 146 ZbAP2/ERF genes that could be classified into 15 subgroups. The motif analysis revealed the presence of different motifs or elements in each group that may explain the functional differences between the groups. ZbERF13.2, ZbRAP2-12, and ZbERF2.1 showed high levels of expression in the early stages of fruit development. ZbRAP2-4, and ZbERF3.1 were significantly expressed at the fruit coloring stage (R2 and G2). ZbERF16 were significantly expressed at fruit ripening and expression level increased as the fruit continued to develop. Relative gene expression levels of 6 representative ZbAP2/ERFs assessed by RT-qPCR agreed with transcriptome analysis results.
CONCLUSIONS: These genes identified by screening can be used as candidate genes that affect fruit development. The results of the analysis can help guide future genetic improvement of Chinese prickly ash and enrich our understanding of AP2/ERF transcription factors and their regulatory functions in plants.
摘要：
背景：AP2/ERF是植物转录因子蛋白的一个大家族，在信号转导中起着必不可少的作用，植物生长发育,以及对各种压力的反应。AP2/ERF家族已在各种植物中通过功能分析进行了鉴定和验证，但是到目前为止，还没有对花椒中这些因素进行全面的研究。系统发育,主题,本研究对花椒不同发育阶段(花后30、60和90天)的果实进行了功能分析和转录组分析。
结果：分析确定了146个ZbAP2/ERF基因，可分为15个亚组。基序分析显示每个组中存在不同的基序或元素，这可以解释组之间的功能差异。ZbERF13.2，ZbRAP2-12和ZbERF2.1在果实发育的早期阶段显示出高水平的表达。ZbRAP2-4和ZbERF3.1在果实着色期(R2和G2)显著表达。ZbERF16在果实成熟时显著表达,并且随着果实继续发育,表达水平增加。通过RT-qPCR评估的6个代表性ZbAP2/ERF的相对基因表达水平与转录组分析结果一致。
结论：通过筛选鉴定的这些基因可用作影响果实发育的候选基因。分析结果有助于指导花椒的遗传改良，丰富我们对植物AP2/ERF转录因子及其调控功能的认识。
结果：分析确定了146个ZbAP2/ERF基因，可分为15个亚组。基序分析显示每个组中存在不同的基序或元素，这可以解释组之间的功能差异。ZbERF13.2，ZbRAP2-12和ZbERF2.1在果实发育的早期阶段显示出高水平的表达。ZbRAP2-4和ZbERF3.1在果实着色期(R2和G2)显著表达。ZbERF16在果实成熟时显著表达,并且随着果实继续发育,表达水平增加。通过RT-qPCR评估的6个代表性ZbAP2/ERF的相对基因表达水平与转录组分析结果一致。
结论：通过筛选鉴定的这些基因可用作影响果实发育的候选基因。分析结果有助于指导花椒的遗传改良，丰富我们对植物AP2/ERF转录因子及其调控功能的认识。
