Abstract：
The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling is activated by infections of bacteria, fungi, viruses and parasites and mediated cellular and humoral immune responses. In the pea aphid Acyrthosiphon pisum little is known about the function of JAK/STAT signaling in its immune system. In this study, we first showed that expression of genes in the JAK/STAT signaling, including the receptors Domeless1/2, Janus kinase (JAK) and transcriptional factor Stat92E, is up-regulated upon bacteria Escherichia coli and Staphylococcus aureus and fungus Beauveria bassiana infections. After knockdown of expression of these genes by means of dsRNA injection, the aphids harbored more bacteria and suffered more death after infected with E. coli and S. aureus, but showed no significant change after B. bassiana infection. Our study suggests the JAK/STAT signaling contributes to the defense against bacterial infection in the pea aphid.
摘要：
Janus激酶/信号转导和转录激活因子（JAK/STAT）信号被细菌感染激活，真菌,病毒和寄生虫以及介导的细胞和体液免疫反应。在豌豆蚜虫中，对JAK/STAT信号在其免疫系统中的功能知之甚少。在这项研究中,我们首先证明了JAK/STAT信号中基因的表达，包括受体Domeless1/2，Janus激酶（JAK）和转录因子Stat92E，在细菌大肠杆菌和金黄色葡萄球菌和真菌白僵菌感染时上调。通过dsRNA注射敲除这些基因的表达后，蚜虫携带更多的细菌，感染大肠杆菌和金黄色葡萄球菌后死亡更多，但感染后无明显变化。我们的研究表明JAK/STAT信号有助于防御豌豆蚜虫的细菌感染。
