Abstract：
BACKGROUND: Dried blood spots (DBSs) collected and archived in newborn screening programs (NSP) represent a potentially valuable resource for assessing exposure to a range of organic and inorganic chemicals in newborns. This study develops and optimizes a method to measure polychlorinated naphthalenes (PCNs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), and organochlorine pesticides (OCPs) in DBS using the isotope dilution technique, ultrasonic-assisted liquid-liquid extraction, simple cleanup, triple quadrupole GC-MS/MS analysis, and background correction.
RESULTS: We minimize the number of extraction repetitions and the volume of solvent, which helps increase throughput while minimizing the potential for contamination. We obtained high recovery and precision for most compounds, and method detection limits (MDLs) were sufficiently low to detect the more prevalent compounds based on representative sample of the US population. MDLs averaged 0.020 ng/mL (recovery: 107 %, precision: 4 %) for PCNs, 0.021 ng/mL (recovery: 97 %, precision: 4 %) for PCBs, 0.021 ng/mL (recovery: 117 %, precision: 2 %) for OCPs, and 0.021 ng/mL (recovery: 96 %, precision: 3 %) for PBDEs.
UNASSIGNED: To our knowledge, this is the first study presenting an analytical method and for PCNs in DBS, and one of the few studies providing an assessment of method performance for persistent organic pollutants in DBS. The optimized method can be applied to a wide range of applications, including exposure assessment, environmental epidemiology, forensics, environmental surveillance, and ecological monitoring.
RESULTS: We minimize the number of extraction repetitions and the volume of solvent, which helps increase throughput while minimizing the potential for contamination. We obtained high recovery and precision for most compounds, and method detection limits (MDLs) were sufficiently low to detect the more prevalent compounds based on representative sample of the US population. MDLs averaged 0.020 ng/mL (recovery: 107 %, precision: 4 %) for PCNs, 0.021 ng/mL (recovery: 97 %, precision: 4 %) for PCBs, 0.021 ng/mL (recovery: 117 %, precision: 2 %) for OCPs, and 0.021 ng/mL (recovery: 96 %, precision: 3 %) for PBDEs.
UNASSIGNED: To our knowledge, this is the first study presenting an analytical method and for PCNs in DBS, and one of the few studies providing an assessment of method performance for persistent organic pollutants in DBS. The optimized method can be applied to a wide range of applications, including exposure assessment, environmental epidemiology, forensics, environmental surveillance, and ecological monitoring.
摘要：
背景：在新生儿筛查计划（NSP）中收集和存档的干血斑（DBS）代表了评估新生儿暴露于一系列有机和无机化学物的潜在有价值的资源。本研究开发并优化了一种测量多氯萘（PCN）的方法，多氯联苯（PCBs），多溴联苯醚（PBDEs），和有机氯农药（OCPs）在DBS中使用同位素稀释技术，超声波辅助液-液萃取,简单的清理，三重四极杆GC-MS/MS分析,和背景校正。
结果：我们将提取重复次数和溶剂体积降至最低，这有助于增加吞吐量，同时最大限度地减少污染的可能性。我们获得了大多数化合物的高回收率和精密度，和方法检测限(MDL)足够低,足以检测基于美国人群代表性样本的更普遍的化合物.MDL平均0.020ng/mL(回收率：107%，精度：4%)用于PCN，0.021ng/mL（回收率：97％，精度：4%)用于PCB，0.021ng/mL（回收率：117%，OCP的精度：2%)，和0.021ng/mL（回收率：96%，PBDEs的精度：3%）。
■据我们所知，这是第一个提出分析方法和DBS中PCN的研究，也是对DBS中持久性有机污染物的方法性能进行评估的少数研究之一。该优化方法可应用于广泛的应用,包括暴露评估，环境流行病学，取证,环境监测,生态监测。
结果：我们将提取重复次数和溶剂体积降至最低，这有助于增加吞吐量，同时最大限度地减少污染的可能性。我们获得了大多数化合物的高回收率和精密度，和方法检测限(MDL)足够低,足以检测基于美国人群代表性样本的更普遍的化合物.MDL平均0.020ng/mL(回收率：107%，精度：4%)用于PCN，0.021ng/mL（回收率：97％，精度：4%)用于PCB，0.021ng/mL（回收率：117%，OCP的精度：2%)，和0.021ng/mL（回收率：96%，PBDEs的精度：3%）。
■据我们所知，这是第一个提出分析方法和DBS中PCN的研究，也是对DBS中持久性有机污染物的方法性能进行评估的少数研究之一。该优化方法可应用于广泛的应用,包括暴露评估，环境流行病学，取证,环境监测,生态监测。
