Abstract：
Paper mulberry (Broussonetia papyrifera) is a fast-growing tree known for its tolerance to diverse biotic and abiotic stresses. To explore genes combating Verticillium wilt, a devasting and formidable disease damage to cotton and many economically significant crops, we purified an antifungal protein, named BpAFP, from the latex of paper mulberry. Based on peptide fingerprint, we cloned the full cDNA sequence of BpAFP and revealed that BpAFP belongs to Class I chitinases, sharing 74 % identity with B. papyrifera leaf chitinase, PMAPII. We further introduced BpAFP into Arabidopsis, tobacco, and cotton. Transgenic plants exhibited significant resistance to Verticillium wilt. Importantly, BpAFP also demonstrated insecticidal activity against herbivorous pests, Plutella xylostella, and Prodenia litura, when feeding the larvae with transgenic leaves. Our finding unveils a dual role of BpAFP in conferring resistance to both plant diseases and lepidopterous pests.
摘要：
桑树（Broussonetiapapyrifera）是一种快速生长的树，以其对多种生物和非生物胁迫的耐受性而闻名。探索对抗黄萎病的基因，对棉花和许多具有经济意义的作物的毁灭性和可怕的疾病损害，我们纯化了一种抗真菌蛋白,名叫BpAFP,来自纸桑树的乳胶。基于肽指纹图谱，我们克隆了BpAFP的完整cDNA序列，揭示BpAFP属于I类几丁质酶，与B.papyrifera叶几丁质酶共享74%的同一性，PMAPII。我们进一步将BpAFP引入拟南芥，烟草,和棉花。转基因植物对黄萎病表现出明显的抗性。重要的是,BpAFP还表现出对草食性害虫的杀虫活性，小菜蛾,LituraProdenia,当用转基因叶子喂养幼虫时。我们的发现揭示了BpAFP在赋予植物病害和鳞翅目害虫抗性方面的双重作用。
