Abstract：
As a representative gas of food spoilage, the development of rapid hydrogen sulfide (H2S) analysis strategies for food safety control is in great demand. Despite traditional methods for H2S detection possessing great achievements, they are still incapable of meeting the requirement of portability and quantitative detection at the same time. Herein, a nanozyme catalysis pressure-powered sensing platform that enables visual quantification with the naked eye is proposed. In this methodology, Pt nanozyme inherits the catalase-like activity to facilitate the decomposition of H2O2 to O2, which can significantly improve the pressure in the closed container, further pushing the movement of indicator dye. Furthermore, H2S was found to effectively inhibit the catalytic activity of Pt nanozyme, indicating that the catalase-like activity of PtNPs may be regulated by varying concentrations of H2S. Therefore, by utilizing a self-designed pressure-powered microchannel device, the concentration of H2S was successfully converted into a distinct signal variation in distance. The effectiveness of the as-designed sensor in assessing the spoilage of red wine by H2S determination has been demonstrated. It exhibits a strong correlation between the change in dye distance and H2S concentration within the range of 1-250 μM, with a detection limit of 0.17 μM. This method is advantageous as it enhances the quantitative detection of H2S with the naked eye based on the portable pressure-powered sensing platform, as compared to traditional H2S biosensors. Such a pressure-powered distance variation platform would greatly broaden the application of H2S-based detection in food spoilage management.
摘要：
作为食品腐败的代表性气体，开发用于食品安全控制的快速硫化氢（H2S）分析策略的需求很大。尽管传统的H2S检测方法取得了巨大的成就,它们仍然不能同时满足便携性和定量检测的要求。在这里，提出了一种纳米酶催化压力驱动传感平台，该平台可以用肉眼进行视觉量化。在这种方法中，Pt纳米酶继承了过氧化氢酶样活性，促进H2O2分解为O2，可显著提高密闭容器内的压力，进一步推动指示染料的运动。此外，发现H2S有效抑制Pt纳米酶的催化活性，这表明PtNP的过氧化氢酶样活性可能受不同H2S浓度的调节。因此,利用自行设计的压力驱动微通道装置，H2S的浓度成功地转化为一个明显的信号变化的距离。已证明了设计的传感器通过测定H2S来评估红酒腐败的有效性。在1-250μM的范围内，染料距离的变化与H2S浓度之间具有很强的相关性，检测限为0.17μM。这种方法是有利的，因为它增强了基于便携式压力供电传感平台的肉眼对H2S的定量检测，与传统的H2S生物传感器相比。这种压力驱动的距离变化平台将极大地拓宽基于H2S的检测在食品腐败管理中的应用。
