关键词: Vicia faba var minor Antioxidant activity Faba bean Fatty acid LC–MS Phenolic compounds Sugar

来  源:   DOI:10.1007/s12010-024-04993-5

Abstract:
This study explores the nutritional and phytochemical profiling of twenty-three genotypes of Vicia faba L. var. minor seeds cultivated in the experimental field of the Arid Lands Institute of Medenine. Our comprehensive analysis encompasses fatty acid composition, sugar content, phytochemical composition, and antioxidant potential, providing a nuanced understanding of the seeds\' nutritive quality. The investigation revealed substantial variations among genotypes, showcasing the potential for targeted nutritional enhancement. Quantification of total polyphenols, flavonoids, condensed tannins, and radical scavenging activities revealed average values of 16.46 mg GAE/g DW, 6.27 mg CTE/g DW, 0.47 mg CE/g DW, and 0.146 mM TEAC, respectively. Notably, the seeds exhibited a low tannin content, a desirable trait for animal feed applications. Liquid chromatography-mass spectrometry (LC-MS) was employed for the identification of phenolic compounds, unearthing the prevalence of quinic acid and flavanols, including catechin (+) and epicatechin. Sugar analysis identified the presence of glucose and sucrose, emphasizing the seeds\' unique carbohydrate composition. Gas chromatography elucidated the fatty acid profile, spotlighting prominent components such as palmitic acid (13.87%), stearic acid (3.37%), oleic acid (27.66%), linoleic acid (45.83%), and linolenic acid (3.53%). The findings underscore the seeds\' nutritive significance, positioning them as rich sources of natural antioxidants, fatty acids, and phenolic compounds. Moreover, the extracts\' favorable tannin content positions them as potential candidates for functional food applications, showcasing their promise as sources of bioactive molecules with diverse applications.
摘要:
本研究探讨了23种基因型蚕豆的营养和植物化学分析。在Medenine干旱土地研究所的试验田中种植的次要种子。我们的全面分析包括脂肪酸组成,含糖量,植物化学成分,和抗氧化潜力,提供对种子营养品质的细微差别的理解。调查显示基因型之间存在很大差异,展示有针对性的营养增强的潜力。总多酚的定量,黄酮类化合物,缩合单宁,自由基清除活性显示平均值为16.46mgGAE/gDW,6.27mgCTE/gDW,0.47mgCE/gDW,和0.146mMTEAC,分别。值得注意的是,种子显示出低单宁含量,动物饲料应用的理想性状。液相色谱-质谱(LC-MS)用于酚类化合物的鉴定,发掘奎尼酸和黄烷醇的流行,包括儿茶素(+)和表儿茶素。糖分析确定了葡萄糖和蔗糖的存在,强调种子独特的碳水化合物成分。气相色谱法阐明了脂肪酸谱,突出成分,如棕榈酸(13.87%),硬脂酸(3.37%),油酸(27.66%),亚油酸(45.83%),和亚麻酸(3.53%)。这些发现强调了种子的营养意义,将它们定位为天然抗氧化剂的丰富来源,脂肪酸,和酚类化合物。此外,提取物\'有利的单宁含量将它们定位为功能性食品应用的潜在候选者,展示了他们作为生物活性分子来源的前景,具有不同的应用。
公众号