Abstract：
Nanostructured lipid carriers (NLC) have low storage and gastrointestinal stability, limiting their applicability. The work aimed to elevate the stability and behaviour of NLC in the alimentary tract by creating an alginate bead. Through the extrusion dropping procedure, Resveratrol (RES)-loaded NLC were efficiently integrated into alginate beads. The incorporation had no significant impact on the particle size, morphology, or inner structure of NLC, as assessed using DLS (Dynamic Light Scattering), SEM (Scanning Electron Microscopy), Differential Scanning Calorimetry (DSC) and FT-IR (Fourier Transform Infra-Red). Incorporating NLC into alginate beads improves its physical stability compared to dispersion of NLC as well as NLC-Sol. An in vitro release investigation found that the NLC-alginate beads released RES more slowly than optimized NLC formulation (RES-NLCs-opt) and NLC-alginate sol. Research on simulated in vitro digestive models revealed that just a small amount of integrated NLC may permeate stomach fluid due to its tiny size. The slow diffusion of NLC from alginate to intestinal fluid prevented aggregation and allowed for gentle hydrolysis of the lipid matrix. Incorporating NLC in alginate beads shows promise for improving stability, modifying gastrointestinal behaviour, and controlling release throughout the process of digestion.
摘要：
纳米结构脂质载体(NLC)具有较低的储存和胃肠道稳定性,限制其适用性。该工作旨在通过产生藻酸盐珠来提高消化道中NLC的稳定性和行为。通过挤压滴落程序，将负载白藜芦醇(RES)的NLC有效地整合到藻酸盐珠粒中。掺入对粒径没有显著影响，形态学,或NLC的内部结构，使用DLS(动态光散射)评估，扫描电子显微镜(SEM),差示扫描量热法(DSC)和FT-IR(傅里叶变换红外)。与NLC以及NLC-Sol的分散体相比,将NLC掺入藻酸盐珠粒中改善了其物理稳定性。体外释放研究发现,NLC-藻酸盐珠释放RES比优化的NLC制剂(RES-NLC-opt)和NLC-藻酸盐溶胶更慢。对模拟体外消化模型的研究表明，由于其微小的尺寸，只有少量的整合NLC可以渗透胃液。NLC从藻酸盐到肠液的缓慢扩散防止了聚集并允许脂质基质的温和水解。在藻酸盐珠粒中掺入NLC显示出改善稳定性的希望，改善胃肠道行为，并在整个消化过程中控制释放。
