Abstract：
One of the most important areas of nanotechnology is the use of nanoparticles (NPs) for a variety of environmental and biological applications, with silver nanoparticles (Ag-NPs) gaining a lot attention due to their distinct properties. The current study deals with the synthesis of Ag-NPs from Dicliptera bupleuroides Nees leaf extract and to determine their antioxidant, antimicrobial potential and effects on wheat seed germination and growth. UV-Visible spectrum revealed a prominent absorption peak at 442 nm, elucidating the conformation of the Ag-NPs synthesis. Scanning electron microscopy (SEM) showed distinctive triangular, pyramidal, and irregular shape. X-ray diffraction (XRD) demonstrated their crystalline nature, with average crystallite size of the Ag-NPs measured at 20.52 nm. Fourier-transform infrared spectroscopy (FT-IR) further confirmed the presence of functional groups such as Phenols (O-H stretch), transition metal carbonyls N-H, ≡C-H, C ≡ N, C ≡ C, C-Cl, C-Br and O-H bonds on the surface Ag-NPs. The antibacterial activity of the Ag-NPs was most pronounced against Bacillus subtilis, with a zone of inhibition (ZOI) measuring 11 mm ± 0.57 at a concentration of 1000 μg/mL (45% inhibition). Likewise, Ag-NPs exhibited highest antioxidant potential (73.2%) at 100 μg/mL compared with standard (ascorbic acid) which showed (76%) at the same concentration. Furthermore, the effect of D. bupleuroides mediated Ag-NPs on wheat seeds growth and germination was recorded maximum at high concentrations (200-300 ppm). In conclusion, D. bupleuroides mediated Ag-NPs showed safe, cost effective and environmentally friendly synthesis which can be used as an antibacterial and antioxidant agent as well as for enhancing the growth and seed germination of crop seeds globally. RESEARCH HIGHLIGHTS: Nanotechnology is the study of nanoparticles for biological and environmental applications. Ag-NPs among other NPs have received broad attention because of their unique properties. D. bupleuroides Ag-NPs: 45% antibacterial, 73.2% antioxidant, enhance wheat germination. D. bupleuroides-mediated Ag-NPs are both cost-effective and environmentally beneficial.
摘要：
纳米技术最重要的领域之一是将纳米粒子（NPs）用于各种环境和生物应用，银纳米粒子（Ag-NP）由于其独特的性质而受到广泛关注。目前的研究涉及从双翅目叶提取物中合成Ag-NP，并确定其抗氧化剂，抗微生物潜力及其对小麦种子萌发和生长的影响。紫外可见光谱在442nm处显示出突出的吸收峰，阐明Ag-NP合成的构象。扫描电子显微镜（SEM）显示独特的三角形，锥体,不规则的形状。X射线衍射(XRD)证明了它们的结晶性质,Ag-NP的平均微晶尺寸在20.52nm处测量。傅里叶变换红外光谱（FT-IR）进一步证实了官能团的存在，如酚（O-H拉伸），过渡金属羰基化合物N-H,=C-H,C=N,C=C,C-Cl,表面Ag-NP上的C-Br和O-H键。Ag-NP对枯草芽孢杆菌的抗菌活性最明显，在1000μg/mL(45%抑制)的浓度下,抑制区(ZOI)测量为11mm±0.57。同样，与在相同浓度下显示(76%)的标准品(抗坏血酸)相比,Ag-NP在100μg/mL时显示出最高的抗氧化潜力(73.2%)。此外，在高浓度（200-300ppm）下，柴胡杜鹃花介导的Ag-NP对小麦种子生长和发芽的影响最大。总之,柴胡苷介导的Ag-NP显示安全，具有成本效益和环境友好的合成，可用作抗菌剂和抗氧化剂，以及在全球范围内增强作物种子的生长和种子发芽。研究重点：纳米技术是生物和环境应用纳米粒子的研究。Ag-NP以及其他NP由于其独特的性质而受到广泛关注。柴胡苷Ag-NP：45%抗菌，73.2%抗氧化剂,增强小麦发芽。柴胡D.介导的Ag-NP既具有成本效益又对环境有益。
