Abstract：
Tanacetum nitens ( Boiss. & Noë) Grierson is an aromatic perennial herb used in Turkish traditional medicine to treat headache, fever, and skin diseases. This study aimed to investigate the chemical composition, antioxidant, enzyme inhibition, and cytotoxic properties of T. nitens aerial parts. Organic solvent extracts were prepared by sequential maceration in hexane, dichloromethane, ethyl acetate, and methanol while aqueous extracts were obtained by maceration or infusion. Nuclear magnetic resonance (NMR) and LC-DAD-MS analysis allowed the identification and quantification of different phytoconstituents including parthenolide, tanacetol B, tatridin B, quinic acid derivatives, β-sitosterol, and glycoside derivatives of quercetin and luteolin. The type and amount of these phytochemicals recovered by each solvent were variable and significant enough to impact the biological activities of the plant. Methanolic and aqueous extracts displayed the highest scavenging and ions-reducing properties while the dichloromethane and ethyl acetate extracts exerted the best total antioxidant activity and metal chelating power. Results of enzyme inhibition activity showed that the hexane, ethyl acetate, and dichloromethane extracts had comparable anti-acetylcholinesterase activity and the latter extract revealed the highest anti-butyrylcholinesterase activity. The best α-amylase and α-glucosidase inhibition activities were obtained from the hexane extract. The dichloromethane and ethyl acetate extracts exhibited the highest cytotoxic effect against the prostate carcinoma DU-145 cells. In conclusion, these findings indicated that T. nitens can be a promising source of biomolecules with potential therapeutic applications.
摘要：
硝基苯(Boiss。&Noe)Grierson是一种芳香的多年生草本植物，用于土耳其传统医学治疗头痛，发烧,和皮肤病。本研究旨在研究其化学成分，抗氧化剂，酶抑制，和T.nitens地上部分的细胞毒性。通过在己烷中连续浸渍制备有机溶剂提取物，二氯甲烷,乙酸乙酯,和甲醇，水提取物通过浸渍或输注获得。核磁共振（NMR）和LC-DAD-MS分析允许鉴定和定量不同的植物成分，包括小白菊内酯，tanacetolB,tatridinB,奎尼酸衍生物，β-谷甾醇，以及槲皮素和木犀草素的糖苷衍生物。通过每种溶剂回收的这些植物化学物质的类型和数量是可变的,并且足够显著以影响植物的生物活性。甲醇和水提取物表现出最高的清除和离子还原性能，而二氯甲烷和乙酸乙酯提取物表现出最佳的总抗氧化活性和金属螯合能力。酶抑制活性结果表明,乙酸乙酯,和二氯甲烷提取物具有相当的抗乙酰胆碱酯酶活性，后一种提取物显示出最高的抗丁酰胆碱酯酶活性。从己烷提取物中获得了最佳的α-淀粉酶和α-葡萄糖苷酶抑制活性。二氯甲烷和乙酸乙酯提取物对前列腺癌DU-145细胞表现出最高的细胞毒性作用。总之,这些发现表明T.nitens可能是具有潜在治疗应用的生物分子的有希望的来源。
