PDF(Pubmed)
Abstract：
OBJECTIVE: In this study, first, second, third, and fourth-order derivative spectrophotometric methods utilizing the peak-zero (P-O) and peak-peak (P-P) techniques of measurement were developed for the determination of levofloxacin, norfloxacin, and moxifloxacin. These methods were applied to their combined pharmaceutical dosage form or individually for levofloxacin, norfloxacin, and moxifloxacin.
METHODS: Linearity was established in the concentration range of 2-20 µg/mL. The procedures are simple, quick, and precise. The developed methods are sensitive, accurate, and cost-effective, demonstrating excellent correlation coefficients (R2 = 0.9998) and mean recovery values ranging from 99.20% to 100.08%, indicating a high level of precision.
RESULTS: The developed approach was effectively employed to determine the levofloxacin, norfloxacin, and moxifloxacin content in commercially available pharmaceutical dosages.
CONCLUSIONS: Statistical analysis and recovery tests confirmed the method\'s linearity and accuracy. The results suggest that this method can be utilized for routine analysis in both bulk and commercial formulations. The simplicity, accuracy, and cost-effectiveness of the developed methods make them valuable for pharmaceutical analysis.
METHODS: Linearity was established in the concentration range of 2-20 µg/mL. The procedures are simple, quick, and precise. The developed methods are sensitive, accurate, and cost-effective, demonstrating excellent correlation coefficients (R2 = 0.9998) and mean recovery values ranging from 99.20% to 100.08%, indicating a high level of precision.
RESULTS: The developed approach was effectively employed to determine the levofloxacin, norfloxacin, and moxifloxacin content in commercially available pharmaceutical dosages.
CONCLUSIONS: Statistical analysis and recovery tests confirmed the method\'s linearity and accuracy. The results suggest that this method can be utilized for routine analysis in both bulk and commercial formulations. The simplicity, accuracy, and cost-effectiveness of the developed methods make them valuable for pharmaceutical analysis.
摘要：
目的：在本研究中，首先,第二,第三,开发了利用零峰（P-O）和峰-峰（P-P）测量技术的四阶导数分光光度法测定左氧氟沙星，诺氟沙星,和莫西沙星.这些方法适用于其联合药物剂型或单独用于左氧氟沙星，诺氟沙星,和莫西沙星.
方法：在2-20µg/mL的浓度范围内建立了线性。程序很简单，快,和精确。开发的方法很灵敏，准确,并且具有成本效益，表现出优异的相关系数（R2=0.9998）和平均回收率在99.20％至100.08％之间，表明了很高的精确度。
结果：所开发的方法被有效地用于测定左氧氟沙星，诺氟沙星,和莫西沙星在市售药物剂量中的含量。
结论：统计分析和回收率试验证实了该方法的线性和准确性。结果表明，该方法可用于散装和商业配方的常规分析。简单，准确度,所开发方法的成本效益使其对药物分析有价值。
方法：在2-20µg/mL的浓度范围内建立了线性。程序很简单，快,和精确。开发的方法很灵敏，准确,并且具有成本效益，表现出优异的相关系数（R2=0.9998）和平均回收率在99.20％至100.08％之间，表明了很高的精确度。
结果：所开发的方法被有效地用于测定左氧氟沙星，诺氟沙星,和莫西沙星在市售药物剂量中的含量。
结论：统计分析和回收率试验证实了该方法的线性和准确性。结果表明，该方法可用于散装和商业配方的常规分析。简单，准确度,所开发方法的成本效益使其对药物分析有价值。
