PDF(Pubmed)
Abstract：
Biofuel production by Clostridium acetobutylicum is compromised by strain degeneration due to loss of its pSOL1 megaplasmid. Here we used engineering biology to stably integrate pSOL1 into the chromosome together with a synthetic isopropanol pathway. In a membrane bioreactor continuously fed with glucose mineral medium, the final strain produced advanced biofuels, n-butanol and isopropanol, at high yield (0.31 g g-1), titre (15.4 g l-1) and productivity (15.5 g l-1 h-1) without degeneration.
摘要：
丙酮丁醇梭菌的生物燃料生产由于其pSOL1大质粒的丢失而受到菌株变性的损害。在这里，我们使用工程生物学将pSOL1与合成的异丙醇途径一起稳定地整合到染色体中。在连续添加葡萄糖矿物培养基的膜生物反应器中，最终菌株产生了先进的生物燃料，正丁醇和异丙醇，高产率（0.31gg-1），滴度（15.4gl-1）和生产率（15.5gl-1h-1），无变性。
