Abstract：
In this study, we developed punicalagin-loaded antimicrobial films based on soy protein isolate (SPI) and apple pectin (AP). The AP was derived from apple pomace waste while the punicalagin was obtained from pomegranate peel. Punicalagin was identified to exist in both α- and β-isomers, with the β-type being predominant. The composite films were characterized using scanning electron microscopy, Fourier transformed infrared spectroscopy, X-ray diffraction, and thermogravimetric analysis. Our results demonstrated that the incorporation of AP significantly enhanced the mechanical strength, heat resistance, and barrier properties of the films. Moreover, the composite films integrated with punicalagin exhibited excellent antimicrobial activities against Staphylococcus aureus (with a minimum bactericidal concentration value of 0.25 %), Escherichia coli (with a minimum bactericidal concentration value of 0.50 %), and Aspergillus niger. Finally, these antimicrobial film solutions were tested as coatings on strawberries and found to have significantly better effects on reducing weight loss, improving shelf-life, and maintaining the freshness of strawberries compared to coatings without punicalagin. The results indicate that antimicrobial coatings loaded with punicalagin hold great promise as multifunctional active packaging materials for fruit preservation.
摘要：
在这项研究中,我们开发了基于大豆分离蛋白（SPI）和苹果果胶（AP）的负载punicalagin的抗菌膜。AP来自苹果渣废物，而punicalagin来自石榴皮。Punicalagin被鉴定为存在于α-和β-异构体中，以β型为主。使用扫描电子显微镜对复合膜进行了表征，傅里叶变换红外光谱,X射线衍射,和热重分析。我们的结果表明，AP的掺入显着增强了机械强度，耐热性，和薄膜的阻隔性能。此外，与panicalagin集成的复合膜对金黄色葡萄球菌表现出优异的抗菌活性（最小杀菌浓度值为0.25％），大肠杆菌（最低杀菌浓度值为0.50%），和黑曲霉.最后,这些抗菌膜溶液作为草莓上的涂层进行了测试，发现对减少体重减轻有明显更好的效果，提高保质期，与不含punicalagin的涂料相比，保持草莓的新鲜度。结果表明，负载punicalagin的抗菌涂料有望作为多功能水果保鲜活性包装材料。
